Sample Preparation Methods for Lipidomics Approaches Used in Studies of Obesity.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Ivan LiakhAdriana Mika

Abstract

Obesity is associated with alterations in the composition and amounts of lipids. Lipids have over 1.7 million representatives. Most lipid groups differ in composition, properties and chemical structure. These small molecules control various metabolic pathways, determine the metabolism of other compounds and are substrates for the syntheses of different derivatives. Recently, lipidomics has become an important branch of medical/clinical sciences similar to proteomics and genomics. Due to the much higher lipid accumulation in obese patients and many alterations in the compositions of various groups of lipids, the methods used for sample preparations for lipidomic studies of samples from obese subjects sometimes have to be modified. Appropriate sample preparation methods allow for the identification of a wide range of analytes by advanced analytical methods, including mass spectrometry. This is especially the case in studies with obese subjects, as the amounts of some lipids are much higher, others are present in trace amounts, and obese subjects have some specific alterations of the lipid profile. As a result, it is best to use a method previously tested on samples from obese subjects. However, most of these methods can be also u...Continue Reading

References

Aug 10, 2000·Baillière's Best Practice & Research. Clinical Endocrinology & Metabolism·P Deurenberg, M Yap
Oct 5, 2001·The Journal of Nutrition·E J Parks
Apr 2, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hye-Ran YoonSam-Hyun Cho
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Apr 13, 2004·Medicine and Science in Sports and Exercise·Wouter D van Marken LichtenbeltHarm Kuipers
Jun 3, 2004·The Journal of Clinical Investigation·Soren NielsenMichael D Jensen
Aug 19, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Isabel Bondia-PonsM Carmen López-Sabater
Apr 1, 2006·Journal of Chromatography. a·Mohammad RezaeeSana Berijani
Jul 11, 2006·Methods : a Companion to Methods in Enzymology·Jacek BielawskiAlicja Bielawska
Jul 10, 2007·The American Journal of Clinical Nutrition·Allison M HodgeGraham G Giles
Feb 19, 2008·Journal of Lipid Research·Vitali MatyashDominik Schwudke
Mar 1, 2008·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·C M Chen
Jun 21, 2008·Molecular and Cellular Endocrinology·M DolinkováM Haluzík
Oct 3, 2008·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·W Li, P Rukavina
Jan 28, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Giuseppe Astarita, Daniele Piomelli
Feb 17, 2009·The British Journal of Nutrition·Heather J BlewettCatherine J Field
May 14, 2009·American Journal of Physiology. Endocrinology and Metabolism·Guang YangFahumiya Samad
Jun 16, 2009·Journal of Pharmaceutical and Biomedical Analysis·Alejandro Ruiz-RodriguezElena Ibañez
Jan 23, 2010·Journal of Lipid Research·Romain HarmanceyHeinrich Taegtmeyer
May 25, 2010·Metabolism: Clinical and Experimental·Christian L RothThomas Reinehr
Aug 26, 2010·American Journal of Human Biology : the Official Journal of the Human Biology Council·Gerson PeltzMary K Fadden
Oct 27, 2010·Applied Nursing Research : ANR·Geraldine M BuddKathleen Falkenstein
Feb 19, 2011·Obesity·Anneke M J van HeesEllen E Blaak
May 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Su GaoGary D Lopaschuk
Jun 15, 2011·The Journal of Biological Chemistry·Susumu MitsutakeYasuyuki Igarashi
Nov 24, 2011·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·R S RaoS Kini
Jan 3, 2012·Indian Journal of Clinical Biochemistry : IJCB·P K Nigam
Mar 3, 2012·Circulation Research·William C StanleyKelly A O'Connell
Jul 17, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Janet D SparksKhosrow Adeli
Aug 7, 2012·Journal of Separation Science·Rocio García-VillalbaMar Larrosa
Dec 5, 2012·International Journal of Obesity : Journal of the International Association for the Study of Obesity·J T BrozinickK Grove
Jan 11, 2013·Physiological Reviews·André Tchernof, Jean-Pierre Després
Jun 12, 2013·Lipids·Tomasz SledzinskiJulian Swierczynski

❮ Previous
Next ❯

Methods Mentioned

BETA
fluorescence microscopy

Software Mentioned

RapidTrace

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.