Sample stacking and sweeping in microemulsion electrokinetic chromatography under pH-suppressed electroosmotic flow

Journal of Chromatography. a
Jinhua ZhuZhide Hu

Abstract

Two on-line sample concentration techniques, sample stacking and sweeping under pH-suppressed electroosmotic flow, were evaluated in microemulsion electrokinetic chromatography. The concept of stacking with anion selective electrokinetic injection and a water plug in a reverse-migrating microemulsion (SASIW-RMME) was brought forward in this article. Six flavonoids were concentrated using a microemulsion consisting of 80 mM sodium dodecyl sulfate, 1.2% (v/v) ethyl acetate, 0.6% (v/v) 1-butanol, 10% acetonitrile (v/v) and 50 mM phosphoric acid (pH* 1.8). Significant detector response improvements were achieved. The limits of detection were in the low ng/ml level. Finally, the sample of Fructus aurantii Immaturus was analyzed using sweeping technique.

References

Sep 19, 2002·Journal of Chromatography. a·Joselito P QuirinoShigeru Terabe
Jan 3, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Patricia PuigMarta Calull

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Citations

Mar 4, 2009·Analytical and Bioanalytical Chemistry·Agnes T AranasJoselito P Quirino
Jun 27, 2013·Journal of the Science of Food and Agriculture·Ilona OlędzkaBarbara Pastuszewska
Mar 2, 2010·Electrophoresis·Richie RyanKevin Altria
Dec 21, 2010·Electrophoresis·Richie RyanKevin Altria
Dec 21, 2010·Electrophoresis·Zdena MaláPetr Boček
Dec 21, 2010·Electrophoresis·Sami El DeebRonald Gust
Dec 6, 2011·Electrophoresis·Xiao-jia ChenShao-Ping Li

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