Samples and techniques highlighting the links between obesity and microbiota

Microbial Pathogenesis
Emmanouil Angelakis, Jean-Christophe Lagier

Abstract

The composition of gut microbiota and its relationship to human health, particularly its links with obesity remain an ongoing challenge for scientists. The current gold standard for exploring human gut microbiota consists of using stool samples and only applying next generations sequencing techniques, which sometimes generate contradictory results. Here, we comprehensively describe nutrient absorption, fat digestion, carbohydrate and protein absorption, demonstrating that absorption of these diverse nutrients occurs mainly in the stomach and small intestine. Indeed, bariatric surgery, including Roux-en-Y, removes part of the upper intestine, resulting in weight loss, while colonic surgery is associated with a stable weight. However, most studies only use stool samples rather than small intestine samples because of the easy with which this can be accessed. Metagenomics studies are associated with several biases such as extraction and primer biases and depth bias, including the more modern platforms. High-throughput culture-dependent techniques, such as culturomics, which uses rapid identification methods such as MALDI-TOF, remain time-consuming, but have demonstrated their complementarity with molecular techniques. In conclusion...Continue Reading

References

Jan 1, 1978·The Journal of Infectious Diseases·P CorrodiS M Finegold
Mar 22, 1979·The New England Journal of Medicine·M H Sleisenger, Y S Kim
Apr 28, 1983·The New England Journal of Medicine·M P HockingE R Woodward
Apr 9, 1981·The New England Journal of Medicine·I H AndersonM D Levitt
May 21, 1999·Science·J W CostertonE P Greenberg
Apr 7, 2004·Science·J Craig VenterHamilton O Smith
Apr 16, 2005·Science·Paul B EckburgDavid A Relman
Jun 3, 2006·Science·Steven R GillKaren E Nelson
Sep 28, 2006·Annual Review of Physiology·David Q-H Wang
Dec 22, 2006·Nature·Ruth E LeyJeffrey I Gordon
Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daniel N FrankNorman R Pace
Oct 19, 2007·Nature·Peter J TurnbaughJeffrey I Gordon
Dec 7, 2007·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Elena ValassiFrancesco Cavagnini
Feb 2, 2008·Environmental Microbiology·Pauline D ScanlanJulian R Marchesi
Dec 2, 2008·Nature·Peter J TurnbaughJeffrey I Gordon
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Husen ZhangRosa Krajmalnik-Brown
Feb 7, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Sami OikarinenHeikki Hyöty
Feb 19, 2009·The Journal of Infectious Diseases·Cyrille J BonhommeDidier Raoult
Apr 25, 2009·Obesity·Arlette SantacruzUNKNOWN EVASYON Study Group
Apr 7, 2010·Proceedings of the National Academy of Sciences of the United States of America·Peter J TurnbaughJeffrey I Gordon
May 22, 2010·FEMS Microbiology Letters·Sonia R VartoukianWilliam G Wade
Nov 26, 2010·Current Opinion in Gastroenterology·Paul D Cotter
Dec 29, 2010·International Journal of Inflammation·Gerald W Tannock
Mar 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Andrew L GoodmanJeffrey I Gordon
Mar 31, 2011·World Journal of Gastroenterology : WJG·Hao-Jiang ZuoXiao-Fang Pei
Apr 22, 2011·Nature·Manimozhiyan ArumugamPeer Bork
Aug 11, 2011·International Journal of Obesity : Journal of the International Association for the Study of Obesity·M MillionD Raoult
Dec 24, 2011·Future Microbiology·Emmanouil AngelakisDidier Raoult
Feb 1, 2010·Nutrients·Jeffrey S CohnSally Tandy
Mar 1, 2012·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·Rosa Krajmalnik-BrownJohn K DiBaise
Mar 16, 2012·Journal of Food Science·Emmanouil AngelakisDidier Raoult
Apr 11, 2012·Molecular Ecology·Shadi ShokrallaMehrdad Hajibabaei
Jun 22, 2012·The British Journal of Nutrition·Ana Paula Boroni MoreiraRita de Cássia Gonçalves Alfenas
Jul 4, 2012·Journal of Applied Microbiology·C SchraderR Johne
Jul 27, 2012·Therapeutic Delivery·Jean-Claude Bakala N'GomaFrédéric Carrière
Aug 29, 2012·Nature Reviews. Gastroenterology & Hepatology·Judith Aron-WisnewskyKarine Clement

❮ Previous
Next ❯

Citations

Jan 20, 2018·Annual Review of Food Science and Technology·Janina A KrumbeckRobert W Hutkins
Jun 19, 2018·Journal of Clinical Gastroenterology·Gianenrico RizzattiAntonio Gasbarrini
Jan 17, 2017·Scientific Reports·Orlane PatrascuChristel Béra-Maillet
Oct 31, 2019·Clinics·Denis PajeckiMarco Aurélio Santo
Mar 14, 2017·Frontiers in Cellular and Infection Microbiology·Shaocun ZhangHe Huang
Dec 6, 2018·International Journal of Molecular Sciences·Lucrecia Carrera-QuintanarRocio I Lopez-Roa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Frontiers in Cellular and Infection Microbiology
Jean-Christophe LagierDidier Raoult
Nature Reviews. Endocrinology
Jacqueline R Center, Chris P White
© 2021 Meta ULC. All rights reserved