Sampling and analysis of volatile organic compounds in bovine breath by solid-phase microextraction and gas chromatography-mass spectrometry

Journal of Chromatography. a
Jarett P SpinhirneNorbert K Chirase

Abstract

A relatively noninvasive method consisting of a face mask sampling device, solid-phase microextraction (SPME) fibers, and a gas chromatography-mass spectrometry (GC-MS) for the identification of volatile organic compounds (VOCs) in bovine breath was developed. Breath of three morbid steers with respiratory tract infections and three healthy steers were sampled seven times in 19 days for 15 min at each sampling. The breath VOCs adsorbed on the divinylbenzene (DVB)-Carboxen-polydimethyl siloxane (PDMS) 50/30 microm SPME fibers were transported to a laboratory GC-MS system for separation and identification with an in-house spectral library of standard chemicals. A total of 21 VOCs were detected, many of them for the first time in cattle breath. Statistical analyses using Chi-square test on the frequency of detection of each VOC in each group was performed. The presence of acetaldehyde (P < or = 0.05) and decanal (P < or = 0.10) were associated more with clinically morbid steers while methyl acetate, heptane, octanal, 2,3-butadione, hexanoic acid, and phenol were associated with healthy steers at P < or = 0.10. The results suggest that noninvasive heath screening using breath analyses could become a useful diagnostic tool for anima...Continue Reading

References

Feb 15, 1997·Analytical Chemistry·C Grote, Janusz Pawliszyn
Oct 13, 1998·Research in Veterinary Science·D MurphyS Love
Nov 16, 2002·Analytical Chemistry·Heather LordJanusz Pawliszyn

Citations

Jul 25, 2009·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Vincent HarracaP M Guerin
Jun 21, 2011·Environmental Monitoring and Assessment·Urszula KotowskaValery A Isidorov
Sep 24, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Chunhui DengXiangmin Zhang
Jan 29, 2011·Chemical Reviews·Gangfeng OuyangJanusz Pawliszyn
Jun 23, 2006·Proteomics·Neerav D Padliya, Bret Cooper
Sep 28, 2007·Medical and Veterinary Entomology·P Jeanbourquin, P M Guerin
Jul 6, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zhuo-Min ZhangGong-Ke Li
Jan 9, 2015·PLoS Neglected Tropical Diseases·Ryan M YoungE W Cupp
Oct 26, 2013·Analytical Chemistry·Alona BaynHossam Haick
Feb 18, 2010·Sensors and Actuators. B, Chemical·Nicholas StrandCristina E Davis
Dec 2, 2016·Medical and Veterinary Entomology·G G VerocaiT R Unnasch
Dec 28, 2016·International Journal of Molecular Sciences·David J BealeEnzo A Palombo
Apr 13, 2018·Journal of Breath Research·Peter OertelWolfram Miekisch
Oct 3, 2018·Animal : an International Journal of Animal Bioscience·L A GonzálezL O Tedeschi
Jun 4, 2019·Journal of Breath Research·Peter GierschnerWolfram Miekisch
Apr 10, 2008·Journal of Environmental Quality·Ngwa Martin NgwabieTorsten Hinz
Oct 31, 2006·Journal of Environmental Quality·Daniel N Miller, Bryan L Woodbury
Nov 1, 2020·Animals : an Open Access Journal From MDPI·Jacqueline Poldy
Dec 4, 2019·The Science of the Total Environment·Julien KammerBenjamin Loubet

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Nodding Syndrome

Nodding Syndrome is a neurological and epileptiform disorder characterized by psychomotor, mental, and growth retardation. Discover the latest research on Nodding Syndrome here.

LRRK2 & Microtubules

Mutations in the LRRK2 gene are risk-factors for developing Parkinson’s disease (PD). LRRK2 mutations in PD have been shown to enhance its association with microtubules. Here is the latest research.