Sandwich enzyme immunoassay for ornithine decarboxylase

Journal of Immunoassay
M NishiyamaS Hayashi

Abstract

A sensitive enzyme immunoassay (EIA) was developed for the determination of ornithine decarboxylase (ODC, EC 4.1.1.17), in the range of 0.02-10 ng, using an affinity-purified anti-ODC-Fab'-peroxidase conjugate. The amount of ODC protein was determined in crude extracts from the kidney of testosterone-treated mice, regenerating rat liver and human thyroid carcinoma, with purified mouse kidney or rat liver enzyme as standard. In all these tissues, similar activity/protein ratios were found for ODC: 1.2 x 10(6)-1.9 x 10(6) nmol CO2/h/mg of ODC protein, which were roughly equivalent to the final specific activity of purified enzymes. ODC inactivated by alpha- difluoro-methylornithine (DFMO) could also be assayed with this method similarly to active ODC protein. However, ODC-antizyme complex gave a somewhat lower value than free ODC protein.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1988·Preparative Biochemistry·M NishiyamaS Hayashi
Sep 1, 1988·The Biochemical Journal·Y MurakamiS Hayashi
Feb 1, 1985·The Biochemical Journal·Y MurakamiS Hayashi
Jul 16, 1982·Biochimica Et Biophysica Acta·T KamejiS Hayashi

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Citations

Nov 24, 1999·Biochimica Et Biophysica Acta·M SukaY Murakami
Apr 12, 2001·Plant Physiology·P BhatnagarS C Minocha
Aug 20, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Raymond G Schipper, Albert A J Verhofstad
Oct 1, 1990·Clinical Biochemistry·M K Schwartz

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