Sandwich-format ECL immunosensor based on Au star@BSA-Luminol nanocomposites for determination of human chorionic gonadotropin

Biosensors & Bioelectronics
Amin ZhangDaxiang Cui

Abstract

A sandwich-configuration electrochemiluminescence (ECL) immunosensor based on Au star@BSA-Luminol nanocomposites for ultrasensitive determination of human chorionic gonadotropin (HCG) has been developed. In this work, nanostructured Polyaniline hydrogels (Pani) decorated with Pt nanoparticles (Pani/Pt) were utilized to construct the base of this immunosensor, greatly increasing the amount of loaded capture antibodies (Ab1) via linkage reagent glutaraldehyde (GA). The used conducting Pani/Pt nanocomposites possessed the unique features, such as large surface area, high electron transfer speed and favorable electrocatalytic activities of hydrogen peroxide, which offered a prominent platform for this sandwich-sensor and acted as efficient ECL signal amplifier also. Furthermore, we employed horseradish peroxidase (HRP) to block the nonspecific binding sites instead of commonly used bovine serum albumin (BSA), which further amplified the signal of luminol in the present of hydrogen peroxide (H2O2). In addition, Au star@BSA nanocomposites with excellent water solubility, low-toxicity and great biocompatibility were prepared and used to immobilize HCG detection antibodies (Ab2) and luminescent material luminol. Then, the above-synthet...Continue Reading

Citations

Jul 30, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Lina WuXilin Sun
Sep 11, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Ritwik MaityNripen Chanda
Nov 18, 2018·Biosensors & Bioelectronics·Yaqiong GaoSongqin Liu
Jan 1, 2021·Talanta·Julien BiscayLynn Dennany
Nov 5, 2019·Analytical Chemistry·Cheng MaJun-Jie Zhu
Feb 5, 2019·ACS Applied Materials & Interfaces·Marcin DąbrowskiPiyush S Sharma
Feb 18, 2020·ACS Omega·Suchi Mercy GeorgeBalasubramanian Kandasubramanian

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