PMID: 7745246Apr 26, 1995Paper

Sandwich immunoassay for the hapten angiotensin II. A novel assay principle based on antibodies against immune complexes

Journal of Immunological Methods
H TowbinO Zingel

Abstract

Immunoassays for haptens such as short peptides or drugs are usually based on the principle of competition for a limited number of binding sites on antibody molecules. Owing to the small size of these antigens it has been thought that two specific antibodies cannot simultaneously bind a hapten. However, antisera containing so called anti-metatypic antibodies have been reported (Voss et al. (1988) Mol. Immunol. 25, 751-759) that bind to hapten-mAb complexes in a reaction where conformational changes on the primary antibody are important. Here, we report on monoclonal antibody pairs able to form ternary complexes with the octapeptide angiotensin II. The first mAb (mAb1) is conventional and binds angiotensin II with high affinity (Kd 10(-11) M). The secondary (anti-metatypic) mAbs (mAbs2s) recognize the immune complex consisting of angiotensin II bound to mAb1, but only poorly recognize mAb1 alone. An immunization technique involving tolerization with uncomplexed mAb1 was used to generate mAb2s. None of the mAbs2s were able to bind angiotensin II by themselves but all efficiently bound the complex of angiotensin II and mAb1. All mAb2s stabilized the angiotensin II-mAb1 complex and one mAb2 distinctly improved the specificity of th...Continue Reading

References

Oct 1, 1989·Molecular Immunology·E W VossD W Ballard
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Citations

Feb 1, 1996·Current Opinion in Biotechnology·C H Self, D B Cook
Sep 12, 2007·Analytical Chemistry·A González-TecheraG González-Sapienza
Jan 5, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Norihiro KobayashiJunichi Goto
Aug 26, 1998·Clinical Chemistry and Laboratory Medicine : CCLM·C P Price
Jan 20, 2016·Analytical Chemistry·Henri O ArolaTarja K Nevanen
Dec 29, 2015·Analytica Chimica Acta·Huihua QuYan Zhao
May 10, 1996·The Journal of Biological Chemistry·J CarreroE W Voss
Nov 23, 2017·Journal of Natural Medicines·Seiichi SakamotoSatoshi Morimoto
May 13, 2010·Analytical Chemistry·Norihiro KobayashiJunichi Goto

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