PMID: 7544818Aug 18, 1995Paper

Sandwich immunoassay specific for the N-terminal sequence of osteocalcin

Journal of Immunological Methods
H EguchiT Yamaji

Abstract

A monoclonal antibody (10B) against the N-terminal sequence of human osteocalcin was selected to characterize its epitope and species specificity. The cross-reactivity of 10B with human and rat osteocalcin demonstrated that the reactivities of 10B with both human and rat osteocalcins were very similar. The pin technology method was used to determine the epitope and clearly demonstrated that the epitope recognized by 10B was localized to residues 12-16, the sequence of which is identical in rat and human osteocalcin molecules. This monoclonal antibody was found to be useful for designing region-specific sandwich immunoassay systems for human and rat N-terminal osteocalcin (N-OC) molecules using rabbit anti-hN20 and anti-rN20 polyclonal antibodies, respectively. The osteocalcin levels in serum determined by this N-OC method were stable during prolonged storage at 25 degrees C and the method could be usefully applied in the development of immunoassay systems for many osteocalcin molecules from many other species.

References

Jan 1, 1978·Annual Review of Biochemistry·P Y Chou, G D Fasman
May 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·P A PriceN Raman
Jan 1, 1991·Critical Reviews in Clinical Laboratory Sciences·M J Power, P F Fottrell
May 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R P TracyK G Mann
Jan 1, 1983·Calcified Tissue International·G R Mundy, J W Poser

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Citations

Nov 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J T ChenM Shiraki
Sep 1, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M ShirakiY Ouchi
Feb 23, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T MatikainenJ Hellman
May 6, 2003·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·M ShirakiH Orimo

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