Sarafotoxin 6c (S6c) reduces infarct size and preserves mRNA for the ETB receptor in the ischemic/reperfused myocardium of anesthetized rats

Journal of Cardiovascular Pharmacology
Thomas R CrockettCherry L Wainwright

Abstract

The aims of this study were to determine if the ETB receptor agonist, sarafotoxin 6c (S6c) reduces myocardial infarct size following myocardial ischemia and reperfusion and to investigate whether any changes in mRNA for endothelin receptors in the injured myocardium were modified by S6c pretreatment. Hypnorm/Hypnovel anesthetized rats were subjected to occlusion of the left main coronary artery for 30 minutes, followed by 120 minutes reperfusion. Animals were administered a bolus dose of S6c (0.24 nmol kg-1 i.v., n = 10) or saline (n = 15) 5 minutes prior to occlusion. At the end of reperfusion, hearts were stained with Evan's Blue dye to delineate area at risk. A 1.5- to 2.0-mm thick slice was cut transmurally 1 mm below the site of ligation for assessment of infarct size by triphenyltetrazolium chloride. A further transmural slice (2.5-3-mm thick) was cut for assessment of receptor mRNA levels by RTPCR. Administration of S6c caused a transient fall in mean arterial blood pressure (MABP) prior to occlusion and attenuated the fall in MABP induced by coronary occlusion. S6c significantly reduced infarct size (13 +/- 4% of area of slice at risk) compared with control hearts (35 +/- 5%; P < 0.05). In control hearts, there was a ma...Continue Reading

References

Sep 1, 1985·British Journal of Pharmacology·G R BullockC L Wainwright
Jun 1, 1994·Cardiovascular Research·J R Parratt, K A Kane
Jan 1, 1994·Life Sciences·C A SargentG J Grover
Aug 16, 1993·Biochemical and Biophysical Research Communications·K KannoF Marumo
Mar 1, 1996·Journal of Molecular and Cellular Cardiology·P WangM V Cohen
Mar 14, 1998·Circulation·K PönickeO E Brodde
May 22, 1998·Journal of Cardiovascular Pharmacology·T SaitoK Nakao
Dec 23, 1998·Cardiovascular Research·I SharifC L Wainwright
Nov 15, 2000·Journal of Cardiovascular Pharmacology·T R CrockettC L Wainwright
Sep 7, 2002·Stroke; a Journal of Cerebral Circulation·Emelie StenmanLars Edvinsson
Sep 24, 2002·Pharmacology & Therapeutics·P D'Orléans-JusteJ C Honoré

❮ Previous
Next ❯

Citations

Sep 10, 2005·British Journal of Pharmacology·Anthony AllanLindsay Brown
Feb 7, 2006·Pharmacology & Therapeutics·Friedrich BrunnerAdelino F Leite-Moreira
Mar 19, 2014·Life Sciences·Lorenz H LehmannJohannes Backs
Aug 7, 2012·Journal of Pharmacological Sciences·Mamoru OhkitaYasuo Matsumura
May 10, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Heidi L Collins, Stephen E Dicarlo
Aug 8, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Heidi L LujanStephen E DiCarlo
Jan 9, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Heidi L LujanStephen E DiCarlo
Sep 29, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Ryan BoydHui Di Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.