Sarcoidosis in patients with mixed connective tissue disease: clinical, genetic, serological and histological observations

Rheumatology International
Péter SzodorayE Bodolay

Abstract

The objective of this study was to investigate how the development of sarcoidosis influences the disease course of mixed connective tissue disease (MCTD). The cellular composition of MCTD-associated sarcoidosis granulomas was evaluated and also the disease-accompanying T-cell activation and alterations of the serum cytokine levels were measured before and after the therapy. The HLA-DR specific alleles were also assessed. We present two cases with MCTD coexisting sarcoidosis. Serum concentrations of Th1 and Th2 cytokines were assessed by ELISA. Peripheral blood CD3+ total T-cell numbers, CD4+ and CD8+ T-cell subset were determined by flow cytometry. Furthermore, hematoxylin-eosin staining and immunhistochemistry were performed for histological assessment. HLA-DR specific alleles were determined by using PCR-SSP. Elevated number of activated T-cells and high Th1 cytokine levels were detected, mainly IFN-gamma and TNF-alpha. Histologically, CD4+ and CD8+ T-cells were present in the sarcoidosis infiltrations. The haplotypes were to some extent dissimilar from the HLA-DR genotype from patients with MCTD, or sarcoidosis alone. Sarcoidosis enhances the activation of MCTD, based on the laboratory and clinical findings. Our results show...Continue Reading

References

Dec 31, 1997·American Journal of Respiratory and Critical Care Medicine·M BerlinJ Grunewald
Jan 10, 2001·Current Opinion in Rheumatology·J Barnard, L S Newman
Apr 4, 2003·Lancet·Robert P BaughmanRoland M du Bois
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Aug 9, 2005·Rheumatic Diseases Clinics of North America·Ingrid E Lundberg

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Citations

Dec 20, 2008·Current Opinion in Rheumatology·Karina D Torralba, Francisco P Quismorio
Jun 25, 2009·Expert Opinion on Drug Safety·Laura M BurdickAlly-Khan Somani
Aug 11, 2020·Seminars in Respiratory and Critical Care Medicine·W Ennis James
Jan 29, 2016·HLA : Immune Response Genetics·A Paradowska-GoryckaM Jurkowska
Dec 17, 2008·Current Opinion in Rheumatology

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