Sarcolemmal ATP-sensitive potassium channels modulate skeletal muscle function under low-intensity workloads

The Journal of General Physiology
Zhiyong ZhuLeonid V Zingman

Abstract

ATP-sensitive potassium (KATP) channels have the unique ability to adjust membrane excitability and functions in accordance with the metabolic status of the cell. Skeletal muscles are primary sites of activity-related energy consumption and have KATP channels expressed in very high density. Previously, we demonstrated that transgenic mice with skeletal muscle-specific disruption of KATP channel function consume more energy than wild-type littermates. However, how KATP channel activation modulates skeletal muscle resting and action potentials under physiological conditions, particularly low-intensity workloads, and how this can be translated to muscle energy expenditure are yet to be determined. Here, we developed a technique that allows evaluation of skeletal muscle excitability in situ, with minimal disruption of the physiological environment. Isometric twitching of the tibialis anterior muscle at 1 Hz was used as a model of low-intensity physical activity in mice with normal and genetically disrupted KATP channel function. This workload was sufficient to induce KATP channel opening, resulting in membrane hyperpolarization as well as reduction in action potential overshoot and duration. Loss of KATP channel function resulted i...Continue Reading

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Citations

Jan 13, 2016·The Journal of General Physiology·Violetta V KravtsovaIgor I Krivoi
Dec 17, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ekaterina SubbotinaLeonid V Zingman
Dec 2, 2015·The Journal of General Physiology·Tarek AmmarJean-Marc Renaud
Feb 5, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Siva Rama Krishna KogantiLeonid Zingman
Feb 2, 2016·Biochemical and Biophysical Research Communications·Ana SierraLeonid V Zingman
Aug 5, 2016·American Journal of Physiology. Cell Physiology·Kyle ScottJean-Marc Renaud
Apr 16, 2020·The Journal of General Physiology·Francine UweraJean-Marc Renaud
Jan 27, 2019·Scientific Reports·Magdalene AmekaMatthew J Potthoff
Apr 23, 2021·Journal of Translational Medicine·Klaus J Wirth, Carmen Scheibenbogen

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Methods Mentioned

BETA
transgenic
dissection
force measurements

Software Mentioned

Nikon NIS Elements AR
pCLAMP
FLIR ResearchIR
Clampex
IDL

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