Sarcoplasmic reticulum interacts with the Ca(2+) indicator precursor fura-2-am

Biochemical and Biophysical Research Communications
S HighsmithK W Snowdowne

Abstract

Fura-2-am, the pentaester precursor of the fluorescent Ca(2+) indicator fura-2, is modified when it is exposed to isolated skeletal muscle sarcoplasmic reticulum vesicles. The modified fura-2-am has enhanced fluorescence, is not sensitive to Ca(2+), and is partially bound to the SR membrane. The isolated product is further converted into fura-2 by esterase. It is suggested that the SR-induced modification is a selective enzymatic hydrolysis of only some of the five ester moieties on fura-2-am. A structure is proposed to account for the results. The potential for this effect of SR on fura-2-am to cause complications in measurements of in vivo intracellular free [Ca(2+)] is noted.

Citations

Jun 1, 1995·Pflügers Archiv : European journal of physiology·J P Chamunorwa, S C O'Neill
Aug 25, 1995·European Journal of Pharmacology·Y HottaJ Sakakibara
Jun 1, 1988·Molecular and Biochemical Parasitology·S A MorrisM Wittner
Feb 1, 1994·Journal of Pharmacological and Toxicological Methods·H Hayashi, H Miyata
Nov 1, 1996·Cellular Signalling·B LorenzW E Müller
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S ChandraG H Morrison
Mar 1, 1994·Journal of Cardiovascular Electrophysiology·A J LeviP Brooksby
Nov 25, 2000·Journal of Applied Physiology·T J HawkeM I Lindinger
Jan 28, 2014·American Journal of Physiology. Heart and Circulatory Physiology·Jennifer K MacDonaldSusan E Howlett
Mar 18, 2020·Cell Calcium·Ana M Rossi, Colin W Taylor
Nov 1, 1987·The American Journal of Physiology·S F SteinbergQ Al-Awqati
Mar 1, 1992·The American Journal of Physiology·H HayashiN Yamazaki
Sep 24, 2005·Journal of Cell Science·Greg FitzHarrisJohn Carroll
Nov 1, 1989·Neurotoxicology and Teratology·S C Bondy
Feb 1, 1990·Cell Calcium·M W RoeB Herman
Mar 1, 1997·Research in Veterinary Science·K E MacEachernA M Nolan

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