PMID: 7529406Dec 11, 1994Paper

Sarkosyl block of transcription reinitiation by RNA polymerase II as visualized by the colliding polymerases reinitiation assay

Nucleic Acids Research
M N Szentirmay, M Sawadogo

Abstract

There are indications that different concentrations of Sarkosyl can block transcription initiation by RNA polymerase II in vitro at different functional steps [Hawley and Roeder (1985) J. Biol. Chem. 260, 8163-8172]. Consequently, this reagent could be a very useful tool for mechanistic studies. So far, however, evidence for the selectivity of Sarkosyl effects on RNA polymerase II transcription has been only indirect. To directly investigate the effect of Sarkosyl on transcription initiation and reinitiation by RNA polymerase II, we employed the reinitiation assay based on utilization of templates containing G-free cassettes (colliding polymerases reinitiation assay, or CoPRA). These experiments showed unambiguously that, under the appropriate conditions, Sarkosyl can be used to block transcription reinitiation by RNA polymerase II while allowing a first round of initiations from preassembled initiation complexes. This inhibition is not due to a disruption of the SII-dependent elongation of the reinitiated transcripts, and the levels of Sarkosyl that prevent transcription reinitiation coincide with the levels that block preinitiation complex assembly. However, Sarkosyl addition to transcription reactions reconstituted with part...Continue Reading

References

Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M N Szentirmay, M Sawadogo
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M Sawadogo, R G Roeder
Aug 30, 1974·FEBS Letters·P GariglioM H Green
Mar 18, 1968·Biochimica Et Biophysica Acta·A Sippel, G Hartmann
Sep 10, 1983·Nucleic Acids Research·S AckermanR Weinmann
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H E TolunayB Safer
Aug 5, 1993·Journal of Molecular Biology·M F LaspiaM B Mathews

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Citations

Sep 6, 2002·Biochimica Et Biophysica Acta·Arik Dvir
Dec 11, 2002·The Journal of Cell Biology·Hiroshi KimuraPeter R Cook
May 21, 1998·Nucleic Acids Research·M N SzentirmayM Sawadogo
Jul 20, 2012·Genes & Development·Andrey RevyakinRobert Tjian
Jan 22, 2011·Journal of Biomedicine & Biotechnology·Sang C LeeCatharine L Smith
Oct 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Donal S Luse
Dec 16, 1998·The Journal of Biological Chemistry·F KashanchiJ N Brady
Jan 1, 2014·Transcription·Giorgio DieciMaria Cristina Bosio
Oct 9, 2019·Proceedings of the National Academy of Sciences of the United States of America·Rina FujiwaraKenji Murakami
Nov 5, 2021·Nature Communications·Zhong ChenQianben Wang

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