SARS-CoV-2 Antibody Responses Are Correlated to Disease Severity in COVID-19 Convalescent Individuals.

The Journal of Immunology : Official Journal of the American Association of Immunologists
Cecilie Bo HansenMikkel-Ole Skjoedt

Abstract

Globally, the COVID-19 pandemic has had extreme consequences for the healthcare system and has led to calls for diagnostic tools to monitor and understand the transmission, pathogenesis, and epidemiology, as well as to evaluate future vaccination strategies. In this study, we have developed novel, to our knowledge, flexible ELISA-based assays for specific detection of human SARS-CoV-2 Abs against the receptor-binding domain, including an Ag sandwich ELISA relevant for large population screening and three isotype-specific assays for in-depth diagnostics. Their performance was evaluated in a cohort of 350 convalescent participants with previous COVID-19 infection, ranging from asymptomatic to critical cases. We mapped the Ab responses to different areas on protein N and S and showed that the IgM, A, and G Ab responses against receptor-binding domain are significantly correlated to the disease severity. These assays and the data generated from them are highly relevant for diagnostics and prognostics and contribute to the understanding of long-term COVID-19 immunity.

References

Apr 12, 2003·The New England Journal of Medicine·Christian DrostenHans Wilhelm Doerr
Mar 24, 2004·Nature Reviews. Microbiology·Konrad StadlerRino Rappuoli
Feb 9, 2008·Emerging Infectious Diseases·Li-Ping WuGuo-Dong Liang
Aug 12, 2014·Viruses·Ruth McBrideBurtram C Fielding
Jun 28, 2016·Nature Reviews. Microbiology·Emmie de WitVincent J Munster
Dec 12, 2018·Nature Reviews. Microbiology·Jie CuiZheng-Li Shi
Feb 23, 2020·Science·Daniel WrappJason S McLellan
Mar 13, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Chuan QinDai-Shi Tian
Mar 30, 2020·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Juanjuan ZhaoZheng Zhang
Apr 3, 2020·The Lancet Infectious Diseases·Robert VerityNeil M Ferguson
Apr 8, 2020·Journal of Advanced Research·Muhammad Adnan ShereenRabeea Siddique
May 16, 2020·Frontiers in Public Health·Jian-Min JinJin-Kui Yang
May 18, 2020·Biochemical and Biophysical Research Communications·Weihong ZengTengchuan Jin
Jun 10, 2020·Nature Reviews. Immunology·Tomer Zohar, Galit Alter
Jul 3, 2020·BMJ : British Medical Journal·Mayara Lisboa BastosFaiz Ahmad Khan
Jul 10, 2020·Nature Communications·Alberto L Garcia-BasteiroCarlota Dobaño
Jul 15, 2020·The New England Journal of Medicine·Lisa A JacksonUNKNOWN mRNA-1273 Study Group
Jul 16, 2020·Journal of Medical Virology·Marie Tré-HardyLaurent Blairon

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Citations

Mar 16, 2021·The Journal of Biological Chemistry·Rafael Bayarri-OlmosMikkel-Ole Skjoedt
Feb 13, 2021·Journal of Clinical Microbiology·Lene H HarritshøjRam B Dessau
May 26, 2021·Human Vaccines & Immunotherapeutics·Natalia T Freund
May 14, 2021·Journal of the European Academy of Dermatology and Venereology : JEADV·M GyldenløveP Garred
Jun 3, 2021·Vaccines·Zacharoula BogogiannidouChristos Hadjichristodoulou
Jul 3, 2021·Vaccine·Kristen A EarleStanley A Plotkin
Jul 1, 2021·Oxford Open Immunology·D Oliver ScourfieldStephanie E A Burnell
Jul 14, 2021·Scientific Reports·John-William Sidhom, Alexander S Baras
Jul 25, 2021·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rafael Bayarri-OlmosPeter Garred
Aug 20, 2021·Laboratory Medicine·Elizabeth Smerczak
Aug 17, 2021·Open Forum Infectious Diseases·Maria Skaalum PetersenPeter Garred
Oct 26, 2021·Frontiers in Immunology·Rafael Bayarri-OlmosMikkel-Ole Skjoedt
Nov 23, 2021·Frontiers in Immunology·Ida JarlheltPeter Garred

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