SARS-CoV-2 D614G variant exhibits efficient replication ex vivo and transmission in vivo.

Yixuan J HouRalph S Baric


The spike aspartic acid-614 to glycine (D614G) substitution is prevalent in global severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains, but its effects on viral pathogenesis and transmissibility remain unclear. We engineered a SARS-CoV-2 variant containing this substitution. The variant exhibits more efficient infection, replication, and competitive fitness in primary human airway epithelial cells but maintains similar morphology and in vitro neutralization properties, compared with the ancestral wild-type virus. Infection of human angiotensin-converting enzyme 2 (ACE2) transgenic mice and Syrian hamsters with both viruses resulted in similar viral titers in respiratory tissues and pulmonary disease. However, the D614G variant transmits significantly faster and displayed increased competitive fitness than the wild-type virus in hamsters. These data show that the D614G substitution enhances SARS-CoV-2 infectivity, competitive fitness, and transmission in primary human cells and animal models.


Jan 31, 2004·Science·UNKNOWN Chinese SARS Molecular Epidemiology Consortium
Feb 7, 2009·Journal of Evolutionary Biology·S AlizonM Van Baalen
Oct 26, 2012·Methods in Molecular Biology·M Leslie Fulcher, Scott H Randell
Nov 5, 2016·Cell·Richard A UrbanowiczJonathan K Ball
Aug 16, 2018·Cell Reports·Michael LetkoVincent Munster
Apr 25, 2020·Nature Medicine·Waradon SungnakUNKNOWN HCA Lung Biological Network
Jun 9, 2020·MedRxiv : the Preprint Server for Health Sciences·Ramon Lorenzo-RedondoEgon A Ozer
Jun 24, 2020·Proceedings of the National Academy of Sciences of the United States of America·Masaki ImaiYoshihiro Kawaoka
Jun 27, 2020·BioRxiv : the Preprint Server for Biology·Lizhou ZhangHyeryun Choe
Aug 4, 2020·BioRxiv : the Preprint Server for Biology·Rachael A MansbachS Gnanakaran
Aug 12, 2020·Journal of Computational Chemistry·I Olaposi OmotuyiKunle Okaiyeto
Aug 28, 2020·Nature·Kenneth H DinnonRalph S Baric
Oct 14, 2020·Proceedings of the National Academy of Sciences of the United States of America·Caitlin E EdwardsRalph S Baric
Oct 18, 2020·MBio·Guillaume Beaudoin-BussièresAndrés Finzi

❮ Previous
Next ❯


Jan 8, 2021·Cell·Wilfredo F Garcia-BeltranAlejandro B Balazs
Jan 14, 2021·Journal of Biomedical Science·Ioannis P TrougakosMeletios A Dimopoulos
Dec 12, 2020·Cell Host & Microbe·Drew WeissmanDavid C Montefiori
Feb 4, 2021·Biology·Santiago Vilar, Daniel G Isom
Jan 30, 2021·Science·Michael A MartinKatia Koelle
Jan 25, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wagner Gouvêa Dos Santos
Feb 16, 2021·Science China. Life Sciences·Aria C Shi, Xuping Xie
Feb 13, 2021·Pathogens·Alejandro Flores-AlanisRosario Morales-Espinosa
Mar 6, 2021·Emerging Microbes & Infections·Kelvin Kai-Wang ToKwok-Yung Yuen
Feb 28, 2021·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Nikolaos I VlachogiannisDimitrios Paraskevis
Mar 7, 2021·Bioengineering·Mark J OsbornJakub Tolar
Mar 11, 2021·Science·Katrina A LythgoeTanya Golubchik
Mar 25, 2021·Scientific Reports·Aysun Urhan, Thomas Abeel
Apr 4, 2021·International Journal of Molecular Sciences·Szu-Wei Huang, Sheng-Fan Wang
Apr 5, 2021·EBioMedicine·Martin S WinklerMarcin F Osuchowski
Apr 6, 2021·Frontiers in Immunology·Jaime Fergie, Amit Srivastava
Apr 16, 2021·The Journal of General Virology·Thomas P PeacockWendy S Barclay
Apr 18, 2021·Science Advances·Rachael A MansbachS Gnanakaran
Apr 25, 2021·Proceedings of the National Academy of Sciences of the United States of America·Phillip PymmWai-Hong Tham
Mar 23, 2021·Expert Review of Clinical Immunology·Rohan AmeratungaAnna E S Brooks
Apr 29, 2021·The Journal of Experimental Medicine·Laura SolforosiRoland C Zahn
Apr 28, 2021·Current Opinion in Virology·Chung-Young Lee, Anice C Lowen

❮ Previous
Next ❯

Methods Mentioned

transmission electron microscopy

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.