Mar 30, 2020

SARS-CoV-2 detection from nasopharyngeal swab samples without RNA extraction

BioRxiv : the Preprint Server for Biology
C. Beltran-PavezGonzalo P. Barriga


The ongoing COVID-19 pandemic has reached more than 200 countries and territories worldwide. Given the large requirement of SARS-CoV-2 diagnosis and considering that RNA extraction kits are in short supply, we investigated whether two commercial RT-qPCR kits were compatible with direct SARS-CoV-2 detection from nasopharyngeal swab samples. We show that one of the tested kits is fully compatible with direct SARS-CoV-2 detection suggesting that omission of RNA extraction step should be considered in SARS-CoV-2 diagnosis.

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Mentioned in this Paper

Establishment and Maintenance of Localization
Transcriptional Regulation
CFC1 gene
Set of Polysomal Ribosomes
Cap Formation
Human Cell Line

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