SARS-CoV-2 diagnostics in the virology laboratory of a University Hospital in Rome during the lockdown period

Journal of Medical Virology
Ombretta TurrizianiGuido Antonelli

Abstract

Italy was one of the most affected nations by coronavirus disease 2019 outside China. The infections, initially limited to Northern Italy, spread to all other Italian regions. This study aims to provide a snapshot of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) epidemiology based on a single-center laboratory experience in Rome. The study retrospectively included 6565 subjects tested for SARS-CoV-2 at the Laboratory of Virology of Sapienza University Hospital in Rome from 6 March to 4 May. A total of 9995 clinical specimens were analyzed, including nasopharyngeal swabs, bronchoalveolar lavage fluids, gargle lavages, stools, pleural fluids, and cerebrospinal fluids. Positivity to SARS-CoV-2 was detected in 8% (527/6565) of individuals, increased with age, and was higher in male patients (P  < .001). The number of new confirmed cases reached a peak on 18 March and then decreased. The virus was detected in respiratory samples, in stool and in pleural fluids, while none of gargle lavage or cerebrospinal fluid samples gave a positive result. This analysis allowed to gather comprehensive information on SARS-CoV-2 epidemiology in our area, highlighting positivity variations over time and in different sex and age group ...Continue Reading

References

Apr 5, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rudragouda ChannappanavarStanley Perlman
Nov 5, 2019·Diagnostic Microbiology and Infectious Disease·I SciandraO Turriziani
Jan 25, 2020·The New England Journal of Medicine·Na ZhuUNKNOWN China Novel Coronavirus Investigating and Research Team
Jan 30, 2020·Viruses·Lisa E Gralinski, Vineet D Menachery
Feb 28, 2020·Journal of Clinical Microbiology·Poramed WinichakoonQuanhathai Kaewpoowat
Feb 29, 2020·The New England Journal of Medicine·Wei-Jie GuanUNKNOWN China Medical Treatment Expert Group for Covid-19
Mar 27, 2020·The Journal of Infection·Zhixiong FangWenhong Zhang
Apr 1, 2020·The Lancet Infectious Diseases·Francois-Xavier LescureYazdan Yazdanpanah
Apr 7, 2020·JAMA : the Journal of the American Medical Association·Giacomo GrasselliUNKNOWN COVID-19 Lombardy ICU Network
May 1, 2020·International Journal of Environmental Research and Public Health·Cosimo DistanteAlessandro Miani
May 1, 2020·Metabolism: Clinical and Experimental·Paolo Pozzilli, Andrea Lenzi
May 1, 2020·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Mohamad NikpouraghdamMahdi Bagheri
May 12, 2020·Cytokine & Growth Factor Reviews·Massimiliano BonafèFabiola Olivieri
May 21, 2020·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Paola MichelozziMarina Davoli

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Citations

Sep 19, 2020·The European Respiratory Journal·José M Porcel
May 11, 2021·The Lancet. Microbe·Guido AntonelliAntonio Angeloni
Jul 3, 2021·Microorganisms·Carla PreziosoValeria Pietropaolo
Aug 2, 2021·Digestive Diseases and Sciences·Giulia ConcasMaria Antonietta Marcialis

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Methods Mentioned

BETA
bronchoalveolar lavage
lavage
Assay

Software Mentioned

GraphPad
GraphPad Prism

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