SARS-CoV-2 escape in vitro from a highly neutralizing COVID-19 convalescent plasma

BioRxiv : the Preprint Server for Biology
Emanuele AndreanoRino Rappuoli

Abstract

To investigate the evolution of SARS-CoV-2 in the immune population, we co-incubated authentic virus with a highly neutralizing plasma from a COVID-19 convalescent patient. The plasma fully neutralized the virus for 7 passages, but after 45 days, the deletion of F140 in the spike N-terminal domain (NTD) N3 loop led to partial breakthrough. At day 73, an E484K substitution in the receptor-binding domain (RBD) occurred, followed at day 80 by an insertion in the NTD N5 loop containing a new glycan sequon, which generated a variant completely resistant to plasma neutralization. Computational modeling predicts that the deletion and insertion in loops N3 and N5 prevent binding of neutralizing antibodies. The recent emergence in the United Kingdom and South Africa of natural variants with similar changes suggests that SARS-CoV-2 has the potential to escape an effective immune response and that vaccines and antibodies able to control emerging variants should be developed. Three mutations allowed SARS-CoV-2 to evade the polyclonal antibody response of a highly neutralizing COVID-19 convalescent plasma.

Citations

Jan 28, 2021·Nature Ecology & Evolution
Feb 12, 2021·International Journal of Molecular Sciences·Bruno O VilloutreixAbdel-Majid Khatib
Feb 25, 2021·Science Immunology·Mrunal SakharkarLaura M Walker
Feb 11, 2021·Nature·Zijun WangMichel C Nussenzweig
Mar 11, 2021·Global Health & Medicine·Douglas D Richman
Feb 16, 2021·AIDS Research and Human Retroviruses·Monty Montano
Mar 17, 2021·Nature Reviews. Immunology·Thiago CarvalhoAkiko Iwasaki
Mar 27, 2021·Nature Medicine·Delphine PlanasOlivier Schwartz
Mar 31, 2021·Virology Journal·Zachary A KrummYuxing Xia
Feb 24, 2021·The Journal of Clinical Investigation·Arturo CasadevallLiise-Anne Pirofski
Mar 12, 2021·Nature·Dami A CollierRavindra K Gupta
Apr 2, 2021·Nature Reviews. Immunology·Sarah CobeyMarc Lipsitch
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna Maria SardanelliLuigi Leonardo Palese
Apr 4, 2021·Viruses·Harry N White
Mar 31, 2021·Environmental Chemistry Letters·Rossana SegretoDaoyu Zhang
Apr 5, 2021·Frontiers in Immunology·Jaime Fergie, Amit Srivastava
Apr 16, 2021·The Journal of General Virology·Thomas P PeacockWendy S Barclay
Apr 14, 2021·Expert Review of Vaccines·Lianlian BianZhenglun Liang
Apr 24, 2021·Science Advances·Annachiara RosaPeter Cherepanov
Apr 27, 2021·Frontiers in Medicine·Vikas BansalRahul Kashyap
May 12, 2021·Emerging Microbes & Infections·Yanan ZhaoDavid S Perlin
May 14, 2021·Clinical Microbiology Reviews·Seyed Hamid Safiabadi TaliSana Jahanshahi-Anbuhi
Jun 3, 2021·International Journal of Molecular Sciences·Stefano BorocciCarmine Talarico
Jun 3, 2021·Nature Reviews. Microbiology·William T HarveyDavid L Robertson
Jun 3, 2021·Journal of Personalized Medicine·Joaquín DopazoManuel Romero-Gómez
Jun 10, 2021·The Journal of Physical Chemistry. B·Nisha BhattaraiPrem P Chapagain

Methods Mentioned

BETA
ELISA

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