SARS-CoV-2 infection dynamics in lungs of African green monkeys.

BioRxiv : the Preprint Server for Biology
Emily SperanzaEmmie de Wit

Abstract

Detailed knowledge about the dynamics of SARS-CoV-2 infection is important for unraveling the viral and host factors that contribute to COVID-19 pathogenesis. Old-World nonhuman primates recapitulate mild-moderate COVID-19 cases, thereby serving as important pathogenesis models. We compared African green monkeys inoculated with SARS-CoV-2 or inactivated virus to study the dynamics of virus replication throughout the respiratory tract. RNA sequencing of single cells from the lungs and mediastinal lymph nodes allowed a high-resolution analysis of virus replication and host responses over time. Viral replication was mainly localized to the lower respiratory tract, with evidence of replication in the pneumocytes. Macrophages were found to play a role in initiating a pro-inflammatory state in the lungs, while also interacting with infected pneumocytes. Our dataset provides a detailed view of changes in host and virus replication dynamics over the course of mild COVID-19 and serves as a valuable resource to identify therapeutic targets.

Citations

Oct 8, 2020·Nature Reviews. Microbiology·Ben HuZheng-Li Shi

❮ Previous
Next ❯

Related Concepts

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

BioRxiv : the Preprint Server for Biology
Emily SperanzaEmmie de Wit
BioRxiv : the Preprint Server for Biology
C. K. YindaVincent J Munster
BioRxiv : the Preprint Server for Biology
C. K. YindaVincent J Munster
© 2021 Meta ULC. All rights reserved