SARS-CoV-2 infection induces robust germinal center CD4 T follicular helper cell responses in rhesus macaques.

Research Square
Sonny R. ElizaldiSmita S Iyer

Abstract

CD4 T follicular helper (T fh ) cells are important for the generation of durable and specific humoral protection against viral infections. The degree to which SARS-CoV-2 infection generates T fh cells and stimulates the germinal center response is an important question as we investigate vaccine options for the current pandemic. Here we report that SARS-CoV-2 infection resulted in transient accumulation of pro-inflammatory monocytes and proliferating T fh cells with a T h 1 profile in peripheral blood. CD4 helper cell responses were skewed predominantly toward a T h 1 response in blood, lung, and lymph nodes. We observed the generation of germinal center T fh cells specific for the SARS-CoV-2 spike (S) and nucleocapsid (N) proteins, and a corresponding early appearance of antiviral serum IgG antibodies. Our data suggest that a vaccine promoting T h 1-type T fh responses that target the S protein may lead to protective immunity.

Citations

Oct 20, 2020·Frontiers in Immunology·Jing OuyangYaokai Chen

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Datasets Mentioned

BETA
MT394528

Methods Mentioned

BETA
PMA
density gradient centrifugation
flow cytometry
PCR
ELISA

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