SARS-CoV-2 infection of human iPSC-derived cardiac cells predicts novel cytopathic features in hearts of COVID-19 patients.

BioRxiv : the Preprint Server for Biology
J. A. Perez-BermejoTodd C McDevitt

Abstract

Although COVID-19 causes cardiac dysfunction in up to 25% of patients, its pathogenesis remains unclear. Exposure of human iPSC-derived heart cells to SARS-CoV-2 revealed productive infection and robust transcriptomic and morphological signatures of damage, particularly in cardiomyocytes. Transcriptomic disruption of structural proteins corroborated adverse morphologic features, which included a distinct pattern of myofibrillar fragmentation and numerous iPSC-cardiomyocytes lacking nuclear DNA. Human autopsy specimens from COVID-19 patients displayed similar sarcomeric disruption, as well as cardiomyocytes without DNA staining. These striking cytopathic features provide new insights into SARS-CoV-2 induced cardiac damage, offer a platform for discovery of potential therapeutics, and raise serious concerns about the long-term consequences of COVID-19.

Citations

Sep 25, 2020·Science·Eric J Topol
Sep 2, 2020·Environmental Microbiology·Kenneth TimmisJames Timmis
Oct 15, 2020·Nature Reviews. Immunology·Felix Clemens Richter
Jan 6, 2021·Cells·William P LafuseMurugesan V S Rajaram
Nov 2, 2020·Journal of Translational Medicine·S GrecoF Martelli
Jan 6, 2021·Nature Reviews. Immunology·Oliver Harschnitz, Lorenz Studer
Jan 1, 2021·The Chinese Journal of Physiology·Yu-Hsiu ChangPo-Shiuan Hsieh
Feb 3, 2021·International Journal of Molecular Sciences·Henkie Isahwan Ahmad Mulyadi LaiYi-Ping Yang
Nov 25, 2020·Cells·Ajit Magadum, Raj Kishore
Mar 16, 2021·Current Clinical Microbiology Reports·Christina L BugertMichelle S Parvatiyar
Apr 27, 2021·SN Comprehensive Clinical Medicine·Christina ChanNeil T Wright
Mar 31, 2021·Clinical and Translational Science·Bérénice CharrezKevin E Healy
May 22, 2021·ACS Infectious Diseases·Nathanael D ReynoldsPatricia M Legler

Methods Mentioned

BETA
transmission electron microscopy
ubiquitination

Related Concepts

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.