SARS-CoV-2 Titers in Wastewater Are Higher than Expected from Clinically Confirmed Cases.

MSystems
Fuqing WuEric J Alm

Abstract

Wastewater surveillance represents a complementary approach to clinical surveillance to measure the presence and prevalence of emerging infectious diseases like the novel coronavirus SARS-CoV-2. This innovative data source can improve the precision of epidemiological modeling to understand the penetrance of SARS-CoV-2 in specific vulnerable communities. Here, we tested wastewater collected at a major urban treatment facility in Massachusetts and detected SARS-CoV-2 RNA from the N gene at significant titers (57 to 303 copies per ml of sewage) in the period from 18 to 25 March 2020 using RT-qPCR. We validated detection of SARS-CoV-2 by Sanger sequencing the PCR product from the S gene. Viral titers observed were significantly higher than expected based on clinically confirmed cases in Massachusetts as of 25 March. Our approach is scalable and may be useful in modeling the SARS-CoV-2 pandemic and future outbreaks.IMPORTANCE Wastewater-based surveillance is a promising approach for proactive outbreak monitoring. SARS-CoV-2 is shed in stool early in the clinical course and infects a large asymptomatic population, making it an ideal target for wastewater-based monitoring. In this study, we develop a laboratory protocol to quantify vi...Continue Reading

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Citations

Aug 12, 2020·Transactions of the Royal Society of Tropical Medicine and Hygiene·Anthony D HarriesAjay M V Kumar
Sep 24, 2020·International Journal of Microbiology·Vijaya Nath MishraRameshwar Nath Chaurasia
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Datasets Mentioned

BETA
MT577641.1

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLAST
BLASTN
PMMoV

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