Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first discovered in December 2019 in Wuhan, China, and expeditiously spread across the globe causing a global pandemic. Research on SARS-CoV-2, as well as the closely related SARS-CoV-1 and MERS coronaviruses, is restricted to BSL-3 facilities. Such BSL-3 classification makes SARS-CoV-2 research inaccessible to the majority of functioning research laboratories in the United States; this becomes problematic when the collective scientific effort needs to be focused on such in the face of a pandemic. However, a minimal system capable of recapitulating different steps of the viral life cycle without using the virus' genetic material could increase accessibility. In this work, we assessed the four structural proteins from SARS-CoV-2 for their ability to form virus-like particles (VLPs) from human cells to form a competent system for BSL-2 studies of SARS-CoV-2. Herein, we provide methods and resources of producing, purifying, fluorescently and APEX2-labeling of SARS-CoV-2 VLPs for the evaluation of mechanisms of viral budding and entry as well as assessment of drug inhibitors under BSL-2 conditions. These systems should be useful to those looking to circumvent BSL-3 wo...Continue Reading
References
Nov 2, 1999·Virology·J MaedaS Makino
Feb 7, 2001·Journal of Virology·E Corse, C E Machamer
Mar 6, 2002·The Journal of Experimental Medicine·Sina BavariM Javad Aman
Apr 23, 2002·Journal of Virology·Takeshi NodaYoshihiro Kawaoka
Dec 4, 2003·Nature·Wenhui LiMichael Farzan
Jan 22, 2004·FEMS Immunology and Medical Microbiology·Dana L SwensonM Javad Aman
Nov 20, 2004·Emerging Infectious Diseases·M L NgE E Ooi
Oct 29, 2005·Journal of Virology·Ping-Kun HsiehMing-Fu Chang
Jul 2, 2008·Journal of Biomedical Science·Shui-Mei WangChin-Tien Wang
Aug 30, 2008·Journal of Virology·Y L SiuB Nal
Oct 22, 2010·Virology Journal·Luis M BrancoRobert F Garry
Jan 7, 2011·American Journal of Physiology. Cell Physiology·Kenneth W DunnJohn H McDonald
Oct 23, 2012·Nature Biotechnology·Jeffrey D MartellAlice Y Ting
Feb 28, 2015·Methods in Molecular Biology·Anthony R Fehr, Stanley Perlman
Jul 3, 2015·Journal of Virology·Jason W Westerbeck, Carolyn E Machamer
Jan 24, 2016·Journal of Biomedical Science·Yvonne Ting-Chun YuMing-Fu Chang
Apr 7, 2016·Oncotarget·Chong WangXianzhu Xia
May 19, 2018·Virologica Sinica·Shiyu DaiFei Deng
Feb 6, 2020·Nature·Peng ZhouZheng-Li Shi
Mar 21, 2020·Cell Discovery·Jia LiuManli Wang
Apr 2, 2020·Cell Research·Shuai XiaLu Lu
Jul 10, 2020·Cell·Mehdi BouhaddouNevan J Krogan
Jul 25, 2020·Nature·Laura RivaSumit K Chanda
Aug 28, 2020·Frontiers in Bioengineering and Biotechnology·Ruodan XuNing Li
Sep 15, 2020·Frontiers in Immunology·Hatem Zayed
Mar 19, 2021·Cell Discovery·Jia LiuManli Wang
Citations
May 1, 2021·Cells·Binod KumarTom Gallagher
May 11, 2021·Frontiers in Cellular and Infection Microbiology·Tarun MishraAjit Chande
Jun 3, 2021·Vaccines·Youjun MiJuan Wang
Jul 3, 2021·Pathogens·Zainalabideen A AbdullaMohammad Z Abdulazeez
Jul 3, 2021·International Journal of Molecular Sciences·Lidia V Putlyaeva, Konstantin A Lukyanov
Sep 21, 2021·Faraday Discussions·Viviana Monje-Galvan, Gregory A Voth
Sep 28, 2021·Molecular Biology of the Cell·Monica L Husby, Robert V Stahelin
Sep 29, 2021·MBio·David Bracquemond, Delphine Muriaux
Nov 9, 2021·Frontiers in Microbiology·Antonina NaskalskaKrzysztof Pyrc