SARS-CoV-2 viral load assessment in respiratory samples

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Steven KleiboekerMichelle Altrich

Abstract

Real-time reverse transcriptase polymerase chain reaction (rRTPCR) has been the main method for diagnosis of SARS-CoV-2 infection in the early stages of the COVID-19 pandemic. De-identified results from upper and lower respiratory samples submitted to a reference laboratory demonstrated a positivity rate of 14.9 % (4428 of 29,713 samples tested). Distribution of results by birth year cohort and specimen type suggested general consistency in mean, median and peak values but higher positivity rates in individuals born from 1964 to 1974. Female patients had a significantly lower positivity rate (P < 0.0001), although similar load mean and median values, compared to males. Overall, 15.3 % (676 of 4428 positive results) of positive results had viral loads greater than 8 log10 copies/mL, with occasional samples exceeding 10 log10 copies/mL. These results support quantitative assessment of SARS-CoV-2 viral load in patient testing and efforts to control viral transmission.

Citations

Sep 15, 2020·Biospektrum : Zeitschrift Der Gesellschaft Für Biologishe Chemie (GBCH) Und Der Vereinigung Für Allgemeine Und Angewandte Mikrobiologie (VAAM)·Anja KlussmeierVinzenz Lange
Jan 13, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Byron Freire-Paspuel, Miguel Angel Garcia-Bereguiain
Feb 16, 2021·Frontiers in Medicine·Waleed H MahallawiAbdulmohsen H Al-Zalabani
Nov 16, 2020·Virology Journal·Byron Freire-Paspuel, Miguel Angel Garcia-Bereguiain
Feb 25, 2021·PloS One·Ozlem YarenSteven A Benner
Apr 4, 2021·International Journal of Environmental Research and Public Health·Tareq HusseinMarkku Kulmala
May 1, 2021·Advances in Virology·Jenni VirtanenTarja Sironen
Sep 30, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Ayşegul Karahasan YagciCagatay Cimsit
Jun 25, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Alfredo BrunoMiguel Angel Garcia-Bereguiain
May 2, 2021·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Søren ØrskovLone Simonsen
Aug 18, 2021·Proceedings of the National Academy of Sciences of the United States of America·Jake G CarterTimothy R Dafforn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.