Sarsaparilla (Smilax Glabra Rhizome) extract inhibits migration and invasion of cancer cells by suppressing TGF-β1 pathway

PloS One
Tiantian SheChengchao Shou

Abstract

Sarsaparilla, also known as Smilax Glabra Rhizome (SGR), was shown to modulate immunity, protect against liver injury, lower blood glucose and suppress cancer. However, its effects on cancer cell adhesion, migration and invasion were unclear. In the present study, we found that the supernatant of water-soluble extract from SGR (SW) could promote adhesion, inhibit migration and invasion of HepG2, MDA-MB-231 and T24 cells in vitro, as well as suppress metastasis of MDA-MB-231 cells in vivo. Results of F-actin and vinculin dual staining showed the enhanced focal adhesion in SW-treated cells. Microarray analysis indicated a repression of TGF-β1 signaling by SW treatment, which was verified by real-time RT-PCR of TGF-β1-related genes and immunoblotting of TGFBR1 protein. SW was also shown to antagonize TGF-β1-promoted cell migration. Collectively, our study revealed a new antitumor function of Sarsaparilla in counteracting invasiveness of a subset of cancer cells by inhibiting TGF-β1 signaling.

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Citations

Oct 24, 2012·Toxicology and Applied Pharmacology·Juan LiRen-Wang Jiang
Feb 13, 2018·The American Journal of Chinese Medicine·Shiyao HuaXueyan Fu
Nov 7, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Lisha DongXiaoyu Chen
Jul 30, 2015·Oncotarget·Elizabeth H Corder, Karta Purkh Singh Khalsa
Sep 28, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Prabu MannarreddyKaviyarasan Venkatesan
Feb 18, 2018·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Jian LiangHui-Lian Huang
Nov 30, 2021·Natural Product Research·Bing SunAi-Qun Jia

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