May 23, 2020

SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells

Current Biology : CB
Hindol GuptaTapas K Manna

Abstract

Centrioles are essential components of centrosome, the main microtubule-organizing center of animal cells required for robust spindle bipolarity [1, 2]. They are duplicated once during the cell cycle [3], and the duplication involves assembly of a cartwheel on the pre-existing centriole followed by assembly of triplet microtubules around the cartwheel [4, 5]. Although the molecular details of cartwheel formation are understood [6-13], the mechanisms initiating the formation of centriolar microtubules are not known. Here, we show that the central component of cartwheel, HsSAS-6 plays a crucial role in the formation of centriolar microtubules by interacting with the microtubule nucleation machinery, γ-tubulin ring complex (γ-TuRC) in human cells. The globular N terminus and the central coiled-coil domain of SAS-6 are required for formation of the cartwheel [7, 14], whereas the function of its C-terminal outer cartwheel region in centriole duplication remains unclear. We find that deletion of HsSAS-6 C terminus disrupts microtubule formation in daughter centriole, and as a result, cells fail to form the new centriole. Consequently, this results in mitotic cells having only two centrioles localized at a single site. Detailed molecu...Continue Reading

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Mentioned in this Paper

Gamma-interferon
Microtubule-Organizing Center
Carboxy-Terminal Amino Acid
Molecular Analysis
High Resolution Transmission Electron Microscopy
USP6NL gene
Interferon Gamma Receptor Complex
Centriole Replication
SASS6 protein, human
Central

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