SAT: Free Software for the Semi-Automated Analysis of Rodent Brain Sections With 2,3,5-Triphenyltetrazolium Chloride Staining

Frontiers in Neuroscience
Xiao-Fang ShiFuhong Cai

Abstract

Ischemic stroke places an increasing burden on individuals, families, and societies around the world. However, effective therapies or drugs for ischemic stroke are lacking. Therefore, animal models mimicking ischemic stroke in humans are of great value for preclinical experiments. middle cerebral artery occlusion (MCAO) in mice or rats and subsequent 2,3,5-triphenyltetrazolium chloride (TTC) staining of brain sections are common methods in the study of experimental animal ischemic stroke. In this study, we present and assess the utility of the semi-automated analysis of the TTC staining (SAT) software program, a novel, small, user-friendly, and free software program, in the quantification of the infarct size in rodent brain sections, with TTC staining, by analyzing images captured by cell phones or scan systems. We performed MCAO and TTC staining in adult mice. We then utilized the SAT software and Image J to analyze the infarct size in the brain sections with TTC staining and compared the findings of the two analysis methods. We found that the data on infarct size from SAT and from Image J were comparable, suggesting that the SAT software could be an alternative option to Image J in the evaluation of ischemic stroke.

References

Nov 1, 1991·Stroke; a Journal of Cerebral Circulation·K IsayamaM C Nishimura
Jul 31, 2010·Neuron·Michael A MoskowitzCostantino Iadecola
Jan 27, 2011·Molecular Neurodegeneration·Trent M WoodruffThiruma V Arumugam
Jan 23, 2014·Lancet·Valery L FeiginUNKNOWN Global Burden of Diseases, Injuries, and Risk Factors Study 2010 (GBD 2010) and the GBD Stroke Experts Group
Jun 17, 2015·Pharmacology & Therapeutics·Antonio MorettiRoberto F Villa
Jul 15, 2015·Drug Design, Development and Therapy·Felix FluriChristoph Kleinschnitz
Jan 27, 2016·Circulation·UNKNOWN Writing Group MembersUNKNOWN Stroke Statistics Subcommittee
Jun 4, 2016·Journal of the American College of Cardiology·Priyank KhandelwalRalph L Sacco
Dec 3, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Felix FriedländerRobert Brunkhorst

❮ Previous
Next ❯

Software Mentioned

TTC ( SAT )
Windows
Image
TTC
Image J
SAT )
ScrBar1
Pro Plus
SAT
ScrBar2

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Acute Stroke

A stroke occurs when blood supply to the brain is interrupted depriving the brain of oxygen and nutrients. This feed focuses cerebrovascular accidents including ischemic and paralytic stroke.