Satb1 and Satb2 regulate embryonic stem cell differentiation and Nanog expression.

Genes & Development
Fabio SavareseRudolf Grosschedl

Abstract

Satb1 and the closely related Satb2 proteins regulate gene expression and higher-order chromatin structure of multigene clusters in vivo. In examining the role of Satb proteins in murine embryonic stem (ES) cells, we find that Satb1(-/-) cells display an impaired differentiation potential and augmented expression of the pluripotency determinants Nanog, Klf4, and Tbx3. Metastable states of self-renewal and differentiation competence have been attributed to heterogeneity of ES cells in the expression of Nanog. Satb1(-/-) cultures have a higher proportion of Nanog(high) cells, and an increased potential to reprogram human B lymphocytes in cell fusion experiments. Moreover, Satb1-deficient ES cells show an increased expression of Satb2, and we find that forced Satb2 expression in wild-type ES cells antagonizes differentiation-associated silencing of Nanog and enhances the induction of NANOG in cell fusions with human B lymphocytes. An antagonistic function of Satb1 and Satb2 is also supported by the almost normal differentiation potential of Satb1(-/-)Satb2(-/-) ES cells. Taken together with the finding that both Satb1 and Satb2 bind the Nanog locus in vivo, our data suggest that the balance of Satb1 and Satb2 contributes to the pl...Continue Reading

References

Nov 1, 2001·Annual Review of Cell and Developmental Biology·A G Smith
Apr 5, 2002·Nature·Qi-Long YingAustin G Smith
Oct 11, 2002·Nature·Dag YasuiTerumi Kohwi-Shigematsu
Oct 16, 2002·Annals of the Rheumatic Diseases·E F Wagner
Jan 7, 2003·Genes & Development·Ariel A AvilionRobin Lovell-Badge
Apr 15, 2003·Nature Genetics·Shutao CaiTerumi Kohwi-Shigematsu
Jul 19, 2003·Science·Marie Kmita, Denis Duboule
May 25, 2004·Genes & Development·Séverine Chambeyron, Wendy A Bickmore
Jan 27, 2005·Cloning and Stem Cells·Ian Chambers
Sep 13, 2005·Cell·Laurie A BoyerRichard A Young
Jun 23, 2006·Nature·José SilvaAustin Smith
Jan 11, 2007·Cell Research·Guangjin Pan, James A Thomson
Jan 12, 2007·Development·Hitoshi Niwa
Mar 17, 2007·Nature Reviews. Genetics·Mikhail Spivakov, Amanda G Fisher
Jul 7, 2007·Stem Cells·Amar M SinghNaohiro Terada
Nov 22, 2007·Science·Junying YuJames A Thomson
Dec 22, 2007·Nature·Ian ChambersAustin Smith
Feb 12, 2008·Nature Cell Biology·Jianming JiangHuck-Hui Ng
Feb 20, 2008·Genes & Development·Dana N LevasseurStuart H Orkin
Feb 26, 2008·Cell·George Q Daley, David T Scadden
Sep 20, 2008·Science·Pablo NavarroPhilip Avner
Nov 6, 2008·Cell Stem Cell·Thomas Graf, Matthias Stadtfeld

❮ Previous
Next ❯

Citations

May 14, 2011·Human Genetics·Susanne ArtholdAnton Wutz
Nov 30, 2011·The Journal of Biological Chemistry·Kazuo AsanomaM A Karim Rumi
Jul 13, 2012·Physiological Genomics·Scott A OchsnerNeil J McKenna
Aug 2, 2012·The Journal of Clinical Investigation·Young-Ho AhnJonathan M Kurie
Sep 1, 2012·Diagnostic Pathology·Björn NodinJakob Eberhard
Jul 14, 2010·Stem Cell Research & Therapy·Melinda K Pirity, Andras Dinnyes
Dec 14, 2011·PloS One·Eva BártováVeronika Foltánková
May 19, 2012·PloS One·Konstantin E OrishchenkoSuren M Zakian
Dec 12, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jennie CloseGord Fishell
Jun 19, 2014·International Journal of Hematology·Takafumi Yokota, Yuzuru Kanakura
Sep 21, 2011·The Journal of Cell Biology·Michael Y FessingVladimir A Botchkarev
Sep 10, 2014·Journal of Cellular Physiology·Weijie DongHongbing Jiang
May 21, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Christina I SelingerMaija R J Kohonen-Corish
Aug 19, 2015·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Thomas Dixon-McDougall, Carolyn Brown
Jul 10, 2012·Seminars in Cancer Biology·Terumi Kohwi-ShigematsuYoshinori Kohwi
Dec 27, 2011·Trends in Cell Biology·Jason Wray, Christine Hartmann
Nov 15, 2011·Biochemical and Biophysical Research Communications·Tatsuya IshiguroKoji Okamoto
Jun 11, 2011·Neuroscience Research·Ying HuangYu-Qiang Ding
Jan 15, 2011·Chinese Medical Sciences Journal = Chung-kuo I Hsüeh K'o Hsüeh Tsa Chih·Li LeiLiang Chih-chuan
Jul 6, 2010·Current Opinion in Genetics & Development·Fong Ming KohMiguel Ramalho-Santos
Apr 15, 2014·International Journal of Cancer. Journal International Du Cancer·Anja FrömbergAchim Aigner
Sep 11, 2012·Developmental Biology·Daniel Meulemans Medeiros, J Gage Crump
Apr 9, 2013·Cell Stem Cell·Fabio PucciLea Harrington
Jun 15, 2011·Cell·Stuart H Orkin, Konrad Hochedlinger
Oct 20, 2012·Developmental Cell·Robert NechanitzkyRudolf Grosschedl
Dec 30, 2014·Carcinogenesis·Jason Brocato, Max Costa
Oct 27, 2012·Acta histochemica·Abraham AmsterdamDavid Givol

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.