SATB2 participates in regulation of menadione-induced apoptotic insults to osteoblasts

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Jyh-Ding WeiRuei-Ming Chen

Abstract

Special AT-rich sequence binding protein 2 (SATB2), a nuclear matrix attachment region-binding protein, can regulate embryonic development, cell differentiation, and cell survival. Previous studies showed that SATB2 is involved in osteoblast differentiation and skeletal development. In this study, we evaluated the role of SATB2 in oxidative stress-induced apoptotic insults to human osteoblast-like MG63 cells and mouse MC3T3-E1 cells. Exposure of MG63 cells to menadione increased intracellular reactive oxygen species levels in a concentration- and time-dependent manner. Simultaneously, menadione-induced oxidative stress triggered cell shrinkage and decreased cell viability. In addition, treatment of MG63 cells with menadione time-dependently decreased the mitochondrial membrane potential but enhanced caspase-3 activity. As a result, menadione-induced DNA fragmentation and cell apoptosis. As to the mechanism, exposure of MG63 cells to menadione amplified SATB2 messenger (m)RNA and protein expression in a time-dependent manner. Knockdown of translation of SATB2 mRNA using RNA interference led to chromatin disruption and nuclear damage. When MG63 cells and MC3T3-E1 cells were treated with SATB2 small interfering RNA, menadione-indu...Continue Reading

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Citations

Jan 15, 2014·Cytokine & Growth Factor Reviews·Xiaoying ZhaoXiaoling Zhang
Sep 23, 2014·Archives of Toxicology·Chia-Yu ChangRuei-Ming Chen
Sep 5, 2020·Journal of Cellular and Molecular Medicine·Sanjit K RoyRakesh K Srivastava

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