Satellite cell myogenesis is highly stimulated by the kinase inhibitor iso-H7: comparison with HA1004 and staurosporine effects

Biochemical and Biophysical Research Communications
C LagordI Martelly

Abstract

Differentiation of rat satellite cells, measured by cell fusion into myotubes and isozymic conversion of creatine kinase and phosphoglycerate mutase, was shown to be highly increased in the presence of 1-(5-isoquinolinylsulfonyl)-3-methylpiperazine (iso-H7). This substance inhibited both protein kinase C (PKC) and cAMP-dependent protein kinase (PKA) activities with similar IC50 between 22 and 34 microM. Iso-H7, as well as the PKA inhibitor HA1004 increased myogenic differentiation without altering the proliferation of satellite cells, whereas the proliferation and the differentiation of these cells were inhibited by the PKC inhibitor staurosporine. Our results suggest a predominant negative control of PKA on satellite cell myogenesis.

Citations

Mar 3, 2010·Matrix Biology : Journal of the International Society for Matrix Biology·Isabelle MartellyBruno Constantin
Sep 25, 2003·The Journal of Biological Chemistry·Fabio NaroGeorges Nemoz
Jul 19, 2002·Journal of Biomedical Materials Research·Dulce Papy-GarciaIsabelle Martelly
Nov 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·K M Eyster, D C McFarland
Sep 1, 1993·Neuromuscular Disorders : NMD·C LagordI Martelly

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