Satellite DNA from the brine shrimp Artemia affects the expression of a flanking gene in yeast

Gene
D MaioranoG Badaracco

Abstract

We have previously revealed that in the brine shrimp Artemia franciscana an AluI DNA family of repeats, 113 bp in length, is the major component of the constitutive heterochromatin and that this repetitive DNA shows a stable curvature that confers a solenoidal geometry on the double helix in vitro. It was suggested that this particular structure may play a relevant role in determining the condensation of the heterochromatin. In this report we have cloned hexamers of highly-repetitive sequence (AluI-satellite DNA) in proximity to a yeast lacZ reporter gene on a plasmid. We find that the expression of the reporter gene is affected by the presence of this DNA in a dose- and orientation-dependent manner in the yeast, S. cerevisiae. We show that this effect is not dependent on under-replication or re-arrangements of the repetitive DNA in the cell but is due to decreased expression of the reporter gene. Our results indicate that the AluI-satellite DNA of Artemia per se is able to influence gene expression.

References

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Citations

Mar 8, 2012·Molekuliarnaia biologiia·V V Grechko
Nov 6, 2002·Genome Génome / Conseil National De Recherches Canada·A Martínez-LageJ Méndez
Aug 28, 1999·Genome Génome / Conseil National De Recherches Canada·W L WuT Y Chiang
Jun 30, 2011·Molecular Biology and Evolution·Gustavo C S KuhnJohn S Heslop-Harrison

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