Satellite RNA Increases DNA Damage and Accelerates Tumor Formation in Mouse Models of Pancreatic Cancer

Molecular Cancer Research : MCR
Takahiro KishikawaKazuhiko Koike

Abstract

Highly repetitive tandem arrays such as satellite sequences in the centromeric and pericentromeric regions of chromosomes, which were previously considered to be silent, are actively transcribed in various biological processes, including cancers. In the pancreas, this aberrant expression occurs even in Kras-mutated pancreatic intraepithelial neoplasia (PanIN) tissues, which are precancerous lesions. To determine the biological role of satellite RNAs in carcinogenesis in vivo, we constructed mouse major satellite (MajSAT) RNA-expressing transgenic mice. However, these transgenic mice did not show spontaneous malignant tumor formation under normal breeding. Importantly, however, DNA damage was increased in pancreatic tissues induced by caerulein treatment or high-fat diet, which may be due to impaired nuclear localization of Y-Box Binding Protein 1 (YBX1), a component of the DNA damage repair machinery. In addition, when crossed with pancreas-specific Kras-mutant mice, MajSAT RNA expression resulted in an earlier increase in PanIN formation. These results suggest that aberrant MajSAT RNA expression accelerates oncogenesis by increasing the probability of a second driver mutation, thus accelerating cells to exit from the breakthro...Continue Reading

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Citations

Sep 26, 2019·Frontiers in Molecular Biosciences·Ruth M Densham, Joanna R Morris
Oct 28, 2018·International Journal of Molecular Sciences·Priscilla CascettaCarmine Carbone
Jan 11, 2019·Frontiers in Genetics·Ksenia Smurova, Peter De Wulf
Aug 4, 2019·Scientific Reports·Anjan K SahaRafael Contreras-Galindo

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