Satellitome Analysis in the Ladybird Beetle Hippodamia variegata (Coleoptera, Coccinellidae).

Genes
Pablo MoraPedro Lorite

Abstract

Hippodamia variegata is one of the most commercialized ladybirds used for the biological control of aphid pest species in many economically important crops. This species is the first Coccinellidae whose satellitome has been studied by applying new sequencing technologies and bioinformatics tools. We found that 47% of the H. variegata genome is composed of repeated sequences. We identified 30 satellite DNA (satDNA) families with a median intragenomic divergence of 5.75% and A+T content between 45.6% and 74.7%. This species shows satDNA families with highly variable sizes although the most common size is 100-200 bp. However, we highlight the existence of a satDNA family with a repeat unit of 2 kb, the largest repeat unit described in Coleoptera. PCR amplifications for fluorescence in situ hybridization (FISH) probe generation were performed for the four most abundant satDNA families. FISH with the most abundant satDNA family as a probe shows its pericentromeric location on all chromosomes. This location is coincident with the heterochromatin revealed by C-banding and DAPI staining, also analyzed in this work. Hybridization signals for other satDNA families were located only on certain bivalents and the X chromosome. These satDNAs...Continue Reading

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Citations

Sep 21, 2020·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Vanessa Bellini BardellaDiogo Cavalcanti Cabral-de-Mello
Apr 17, 2021·Frontiers in Genetics·Diogo C Cabral-de-MelloFrantišek Marec
Jul 3, 2021·International Journal of Molecular Sciences·Eugenia E MontielSebastián Pita

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Datasets Mentioned

BETA
PRJNA644199

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

MUSCLE
TAREAN
RepeatExplorer
Adobe ® Photoshop
BLAST
Dotmatcher
Galaxy
calcDivergenceFromAlign
RepeatMasker
RMBlast

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