Satiety induced by bile acids is mediated via vagal afferent pathways.

JCI Insight
Xiaoyin WuChung Owyang

Abstract

The aim of this study was to elucidate the role and the pathways used by bile acid receptor TGR5 in transmitting satiety signals. We showed TGR5 colocalized with cholecystokinin type A (CCK-A) receptors in a subpopulation of rat nodose ganglia (NG) neurons. Intra-arterial injection of deoxycholic acid (DCA) dose-dependently increased firing rate in NG while a subthreshold dose of DCA and CCK-8 increased firing rates synergistically. TGR5-specific agonist oleanolic acid induced NG neuronal firing in a dose-dependent manner. However, the same units did not respond to GW4064, a nuclear receptor-specific agonist. Quantity of DCA-activated neurons in the hypothalamus was determined by c-Fos expression. Combining DCA and CCK-8 caused a 4-fold increase in c-Fos activation. In the arcuate nucleus, c-Fos-positive neurons coexpressed cocaine and amphetamine regulated transcript and proopiomelanocortin. DCA-induced c-Fos expression was eliminated following truncal vagotomy or silencing of TGR5 in the NG. Feeding studies showed intravenous injection of 1 μg/kg of DCA reduced food intake by 12% ± 3%, 24% ± 5%, and 32% ± 6% in the first 3 hours, respectively. Silencing of TGR5 or CCK-A receptor in the NG enhanced spontaneous feeding by 18% ±...Continue Reading

References

Sep 1, 1973·Journal of Comparative and Physiological Psychology·J GibbsG P Smith
Jun 1, 1994·Gastroenterology·R J LieverseC B Lamers
May 21, 1999·Science·M MakishimaB Shan
May 21, 1999·Science·D J ParksJ M Lehmann
Jun 9, 1999·Molecular Cell·H WangB M Forman
Apr 15, 2000·Nature·M W SchwartzD G Baskin
Nov 7, 2002·Biochemical and Biophysical Research Communications·Takaharu MaruyamaKenichi Tanaka
Jan 14, 2003·The Journal of Biological Chemistry·Yuji KawamataMasahiko Fujino
Jan 31, 2003·American Journal of Physiology. Endocrinology and Metabolism·Ira Gantz, Tung M Fong
Jan 1, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Roger D ReidelbergerMartin Hulce
Oct 27, 2006·The Journal of Endocrinology·Takaharu MaruyamaYoshitaka Tamai
Nov 2, 2006·Endocrine Reviews·Roger D Cone
Apr 5, 2007·Nature Protocols·Tetsuichiro Saito
May 13, 2008·Biochemical and Biophysical Research Communications·Verena KeitelDieter Häussinger
May 20, 2008·Cellular and Molecular Life Sciences : CMLS·A F Hofmann, L R Hagey
Sep 26, 2008·Journal of Lipid Research·David W Russell
Jan 8, 2009·Physiological Reviews·Philippe LefebvreBart Staels
Jul 8, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Verena KeitelDieter Häussinger
Dec 15, 2010·Journal of Hepatology·Thijs W H PolsKristina Schoonjans
Jan 5, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shi-Yi ZhouChung Owyang
Sep 1, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Andrea HeldsingerChung Owyang
Oct 2, 2012·Biochemical and Biophysical Research Communications·Divya P KumarArun J Sanyal
Jun 21, 2013·Journal of Receptor and Signal Transduction Research·Vanesa StepanovMomir Mikov
May 6, 2014·Clinical Biochemistry·Tatsuya SuzukiHiroshi Nakano
Oct 30, 2014·Frontiers in Neuroscience·Jackie Lau, Herbert Herzog
Jun 9, 2016·The Journal of Clinical Endocrinology and Metabolism·David P SonneFilip K Knop
Apr 30, 2017·Frontiers in Endocrinology·Jens F Rehfeld
Apr 30, 2017·Medicina·Gintautas Grabauskas, Chung Owyang

❮ Previous
Next ❯

Citations

Jan 11, 2021·Peptides·Carolina R Cawthon, Claire B de La Serre
Apr 23, 2021·Cell Metabolism·Ashley Castellanos-JankiewiczDaniela Cota
Jun 3, 2021·International Journal of Molecular Sciences·Marina Romaní-PérezYolanda Sanz
May 26, 2021·Nature Metabolism·Alessia PerinoKristina Schoonjans
Oct 26, 2021·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Sierra SimpsonOlivier George
Nov 5, 2021·Biological Reviews of the Cambridge Philosophical Society·Ayala GlinertOmry Koren

❮ Previous
Next ❯

Methods Mentioned

BETA
reverse transcription PCR
PCR
Assay
reverse phase chromatography
transfection

Software Mentioned

Spike
Axon

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.