PMID: 6398564Jan 1, 1984Paper

Saturable attachment sites for type 3 mammalian reovirus on murine L cells and human HeLa cells

Virus Research
J R Gentsch, J W Hatfield

Abstract

Attachment of [35S]methionine-labelled mammalian type 3 reovirus to murine L cells and human HeLa cells was studied under equilibrium conditions. Cellular attachment sites could be completely saturated with 35S-labelled reovirus, indicating that specific attachment sites for reovirus are present on the surface of these cells. We calculated that L cells possess about 86000-105000 attachment sites per cell while HeLa cells possess about 126000-147000 sites per cell for type 3 reovirus. Unlabelled reovirus was highly efficient in competing for attachment by 35S-labelled reovirus to the saturable attachment sites of both L and HeLa cells, further indicating the specificity of the interaction. We also found that unlabelled reovirus competed equally well for both binding and internalization of 35S-labelled reovirus into murine L cells, suggesting that the L cell attachment site may serve as a virus entry site. Phospholipase digestion of L cells had no effect on subsequent reovirus attachment, while treatment of L cells with moderate concentrations of bromelain (but not trypsin, proteinase K or pronase) and Vibrio cholerae neuraminidase reproducibly decreased subsequent reovirus attachment. These results and those of others (Epstein e...Continue Reading

References

Oct 1, 1979·The Journal of General Virology·J BorsaJ G Szekely
Jan 1, 1976·Annual Review of Biochemistry·S C SilversteinG Acs
Apr 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R SchlegelI H Pastan
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Feb 1, 1980·The Journal of Cell Biology·A HeleniusE Fries
Jan 15, 1981·Virology·P W LeeW K Joklik
Jan 1, 1968·The Journal of Cell Biology·S C Silverstein, S Dales

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