Saturable CD14-dependent binding of fluorescein-labeled lipopolysaccharide to human monocytes.

Infection and Immunity
A TroelstraJ A van Strijp

Abstract

We used rough lipopolysaccharide (ReLPS) to construct a fluorescein-labeled LPS (FITC-LPS) with a very high labeling efficiency that bound to isolated human monocytes in a CD14-dependent fashion and that in this respect behaved indistinctively from native LPS. The CD14-dependent binding could be inhibited either by a 1,000-fold excess of unlabeled LPS or by polymyxin B, bactericidal/permeability-increasing protein, cationic protein 18, or soluble CD14. Although this FITC-LPS preparation no longer possessed the ability to prime neutrophils for the production of reactive oxygen species or to stimulate human monocytes to produce tumor necrosis factor, activation of the Limulus amoebocyte lysate cascade was comparable to activation by native LPS. Binding to monocytes was enhanced by human pooled serum (HPS) or LPS-binding protein (LBP) for LPS concentrations up to 100 ng/ml and was completely CD14 dependent. For LPS concentrations exceeding 100 ng/ml, binding was still partially CD14 dependent, but not HPS or LBP dependent. CD14-dependent association of LPS with monocytes was shown to be totally saturable. In conclusion, we found an HPS- or LBP-dependent binding of FITC-LPS to monocytes that was CD14 dependent at up to 100 ng of LP...Continue Reading

References

Dec 1, 1992·The Journal of Experimental Medicine·E A FreyS D Wright
Jan 1, 1992·Research in Immunology·C SchüttC Krüger
Aug 1, 1991·Clinical and Experimental Immunology·C KrügerW Schönfeld
Sep 15, 1991·Annals of Internal Medicine·R C Bone
Sep 21, 1990·Science·R R SchumannR J Ulevitch
Sep 1, 1986·The Journal of Experimental Medicine·P S TobiasR J Ulevitch
Dec 1, 1986·European Journal of Immunology·V BazilI Hilgert
Dec 1, 1985·The Biochemical Journal·S J WarnerD Mitchell
Mar 1, 1995·The Journal of Infectious Diseases·R LandmannT Calandra
Jan 1, 1993·Advances in Immunology·R J Ulevitch
Apr 1, 1995·Infection and Immunity·J W LarrickS C Wright
Feb 1, 1997·Journal of Leukocyte Biology·A TroelstraJ A Van Strijp

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Citations

Sep 28, 2002·Current Opinion in Lipidology·Gerd Schmitz, Evelyn Orsó
Aug 29, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jimmy F P BerbéePatrick C N Rensen
Jun 16, 2010·Journal of Periodontal Research·K H ZawawiT E Van Dyke
Apr 9, 2001·Infection and Immunity·J H LevelsS J van Deventer
Jan 29, 2000·European Journal of Clinical Investigation·P Antal-Szalmás
Aug 25, 2009·Journal of Dairy Science·K K M O'DriscollS D Eicher
May 25, 2011·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·András VidaJos A G van Strijp
Apr 23, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Simone B BayerChristine C Winterbourn
Aug 25, 2006·Clinical and Vaccine Immunology : CVI·Mohamed El KhattabiJord Stam
Jul 28, 2005·Journal of Leukocyte Biology·Gabriela C FernándezMarina S Palermo
Dec 18, 2007·European Journal of Immunology·Martha TriantafilouKathy Triantafilou
Mar 29, 2014·Chembiochem : a European Journal of Chemical Biology·Stefania E SestitoFrancesco Peri
Mar 23, 2000·European Journal of Biochemistry·A B SchrommU Seydel
Mar 29, 2001·Nature Immunology·K TriantafilouR L Dedrick
Apr 26, 2001·Microbiology· Tanamoto KS Azumi
Jan 26, 2005·Immunology·Mohamed S ArredouaniJan L Ceuppens
Nov 25, 1998·Biochemical and Biophysical Research Communications·C J de HaasJ A van Strijp
Jun 20, 2002·Trends in Immunology·Martha Triantafilou, Kathy Triantafilou
May 12, 2004·Immunology Letters·Péter Antal-SzalmásJos A G van Strijp

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