Saturated and unsaturated 1-0-alkyl-2-0-acetoyl-sn-glycero-3-phosphocholines and 2-lyso derivatives from ratfish liver oil. Effect on adenosine 3':5'-cyclic monophosphate concentration in hepatocyte suspensions

Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie
I Hillmar, N Zöllner

Abstract

1-0-Alkyl-2-0-acetoyl-sn-glycero-3-phosphocholines, containing saturated or unsaturated alkyl moieties and their 2-lyso derivatives, prepared from ratfish liver oil (Hydrolagus colliei), were studied for their in vitro effect on cellular adenosine 3':5'-cyclic monophosphate (cAMP) concentration in freshly isolated hepatocytes from adult rats. We report here for the first time that semisynthetic platelet-activating factor (PAF), the saturated and unsaturated compound, from 10(-6) M to 10(-12) M, is effective in lowering cAMP concentrations by 50% after 4 h of incubation. This effect becomes apparent after 30 min. Concentrations from 10(-12) M up to 10(-20) M did not influence cAMP levels. The 2-lyso derivatives (10(-8) M) of both compounds are equally active as compared to their 2-0-acetoyl derivatives, in lowering cAMP levels after 4 h of incubation. We discuss the existence of a transacetylating enzyme, generating the active form in rat liver cells.

Citations

Jun 28, 1979·Nature·M ChignardJ Benveniste
Apr 13, 1982·Biochimica Et Biophysica Acta·G H RaoJ G White
Oct 30, 1981·Biochemical and Biophysical Research Communications·T C LeeF Snyder
Mar 16, 1981·Biochemical and Biophysical Research Communications·D J HanahanR N Pinckard

Related Concepts

Fish Liver Oils
1-0-octadecyl 2-0-acetyl sn-glycero-3-phosphorylcholine, (R)-isomer
August Rats
Cyclic AMP, (R)-Isomer
In Vitro [Publication Type]
Metazoa
Liver
Rats, Laboratory
Platelet Activating Factor

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