SC1 sustains the self-renewal capacity and pluripotency of chicken blastodermal cells by inhibiting the phosphorylation of ERK1 and promoting the phosphorylation of Akt

Reproduction in Domestic Animals = Zuchthygiene
Rongyang LiHonglin Liu

Abstract

Small molecules discovered during the recent years can be used to regulate the growth of embryonic stem cells (ES cells). Chicken blastodermal cells (cBCs) play an important role in both basic and transgenic researches as an important ES cell. However, the regulatory mechanism of small molecules involved in the self-renewal and pluripotency of cBCs remains unknown. This study revealed that the small molecule, SC1, can maintain cBCs in an undifferentiated, pluripotent state in serum- and feeder-free E8 media without leukaemia inhibitory factor. Furthermore, SC1 inhibits downregulation of pluripotency-related genes caused by retinoic acid and promotes the proliferation of cBCs. Furthermore, the results of this study indicated that SC1 functions by inhibiting ERK1 phosphorylation and promoting Akt phosphorylation, thus promoting the expression of pluripotency-related genes and maintaining the pluripotency of cBCs. The results also demonstrated that SC1 sustains the self-renewal capacity and pluripotency of cBCs cells by inhibiting ERK1 phosphorylation and promoting Akt phosphorylation. This kind of regulatory mechanism might be conserved in avian ES cells. Other molecules, similar to SC1, might provide insights into the molecular ...Continue Reading

References

Aug 20, 2003·Development·Laura Beth CorsonJanet Rossant
Aug 30, 2005·Nature Biotechnology·Lei ZhuRobert J Etches
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sharoni JacobsRonald M Evans
Nov 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shuibing ChenSheng Ding
Dec 5, 2006·Methods in Enzymology·Marie-Cecile van de Lavoir, Christine Mather-Love
Dec 8, 2006·Nature·Reka R Letso, Brent R Stockwell
Nov 21, 2007·Differentiation; Research in Biological Diversity·Ralph GraichenBruce P Davidson
Aug 13, 2009·Journal of Cell Science·Fumitaka OsakadaMasayo Takahashi
Jan 3, 2013·Development, Growth & Differentiation·Christian JeanBertrand Pain
Jan 24, 2013·PloS One·Christian BuschUlrich Drews
Aug 31, 2013·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Xiaoyan ShiYong Zhang
Jul 20, 2014·International Journal of Cardiology·Yan LiXuesong Yang
Feb 19, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tae Sub ParkBeom Ku Han
Mar 11, 2015·Stem Cell Research·Ben MeadBen A Scheven

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Mar 15, 2019·Current Stem Cell Research & Therapy·Maryam FarzanehSeyed Esmaeil Khoshnam

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