Sca1, a previously undescribed paralog from autotransporter protein-encoding genes in Rickettsia species

BMC Microbiology
Maxime NgwamidibaPierre-Edouard Fournier

Abstract

Among the 17 genes encoding autotransporter proteins of the "surface cell antigen" (sca) family in the currently sequenced Rickettsia genomes, ompA, sca5 (ompB) and sca4 (gene D), have been extensively used for identification and phylogenetic purposes for Rickettsia species. However, none of these genes is present in all 20 currently validated Rickettsia species. Of the remaining 14 sca genes, sca1 is the only gene to be present in all nine sequenced Rickettsia genomes. To estimate whether the sca1 gene is present in all Rickettsia species and its usefulness as an identification and phylogenetic tool, we searched for sca1genes in the four published Rickettsia genomes and amplified and sequenced this gene in the remaining 16 validated Rickettsia species. Sca1 is the only one of the 17 rickettsial sca genes present in all 20 Rickettsia species. R. prowazekii and R. canadensis exhibit a split sca1 gene whereas the remaining species have a complete gene. Within the sca1 gene, we identified a 488-bp variable sequence fragment that can be amplified using a pair of conserved primers. Sequences of this fragment are specific for each Rickettsia species. The phylogenetic organization of Rickettsia species inferred from the comparison of ...Continue Reading

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Citations

Apr 17, 2008·Nature Reviews. Microbiology·David H Walker, Nahed Ismail
Jun 4, 2008·The Pediatric Infectious Disease Journal·Ferran Segura PortaMariela Quesada Vilaseca
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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

MEGA2
ABI Prism DNA sequencing
PAUP
PROTML MOLPHY
paml
SVARAP
Infobiogen
tBLASTn
BLASTn
GenomeWalker

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