Scaffold-free 3D cellulose acetate membrane-based cultures form large cartilaginous constructs

Journal of Tissue Engineering and Regenerative Medicine
Susanne Mayer-WagnerPeter E Müller

Abstract

Scaffold-free three-dimensional (3D) cultures provide clinical potential in cartilage regeneration. The purpose of this study was to characterize a scaffold-free 3D membrane-based culture system, in which human articular chondrocytes were cultivated on a cellulose acetate membrane filter, and compare it to pellet and monolayer cultures. Chondrocytes were expanded in monolayer culture for up to 5 passages, transferred to membrane-based or pellet cultures and harvested after 7 or 21 days. The chondrogenic potential was assessed by histology (toluidine blue, safranin-O), immunohistochemistry for collagen type II and quantitative analysis of collagen type II α(1) (COL2A1). Membrane-based cultures (P1) formed flexible disc-like constructs (diameter 4000 µm, thickness 150 µm) with a large smooth surface after 7 days. Positive safranin-O and collagen type II staining was found in membrane-based and pellet cultures at P1-3. Expression of COL2A1 after 7 days was increased in both culture systems compared to monolayer culture up to P3, whereas cells from monolayer > P3 did not redifferentiate. The best results for COL2A1 expression were obtained from membrane-based cultures at P1. After 21 days the membrane-based cultures did not express...Continue Reading

References

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May 15, 2009·Research in Veterinary Science·Muhammad Farooq RaiMichael F G Schmidt
Sep 1, 2009·Tissue Engineering. Part a·Susanne Mayer-WagnerPeter E Müller

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Citations

Aug 9, 2012·BMC Musculoskeletal Disorders·Markus U WagenhäuserPeter E Müller
Jun 21, 2015·In Vitro Cellular & Developmental Biology. Animal·Seul Ki MinHwa Sung Shin
Jul 21, 2016·Scientific Reports·Yong HeJianZhong Fu
Mar 27, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Justin ParrenoRita A Kandel
Jan 27, 2019·Bioengineering & Translational Medicine·Samson AfewerkiAli Khademhosseini
Apr 23, 2020·International Journal of Molecular Sciences·Nele Pascale GrigullSusanne Mayer-Wagner

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