Scaffold-free cartilage fabrication system using passaged porcine chondrocytes and basic fibroblast growth factor

Tissue Engineering. Part a
Ri Long JinByoung-Hyun Min

Abstract

The scaffold-free cartilage fabrication system we have reported previously for human application with nonpassaged cells has a big limitation in securing the enough cell number. Therefore, autologous chondrocytes from small biopsy of cartilage tissue inevitably have to be expanded through multiple passages, which result in poor engineered cartilage in terms of quality and quantity. This study applied basic fibroblast growth factor (bFGF) to overcome the limitation and produce an engineered cartilage tissue with clinically applicable size and quality. Porcine articular chondrocytes were expanded until passage 2 in the absence or presence of 5 ng/mL bFGF, and then subjected to the two-stage scaffold-free cultures for 21 days again in the absence or presence of 5 ng/mL bFGF. The fabrication of cartilage tissues was evaluated along with time in comparison with the constructs from unpassaged chondrocytes. Expansion of chondrocytes in the presence of bFGF increased accumulation of cartilage extracellular matrices and resultantly fabricated the larger size of cartilage tissues in the subsequent stages, being comparable to those of unpassaged cells. In contrast, bFGF showed no positive, but adverse, effects on the cartilage tissue forma...Continue Reading

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Citations

Dec 1, 2010·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Kazuhiro UenakaYoshitaka Matsusue
Mar 23, 2012·Journal of Tissue Engineering and Regenerative Medicine·Jun ZhuWei Wu
Jan 29, 2011·Biotechnology and Bioengineering·Scott C TranSteven H Elder
Mar 13, 2014·Journal of Biomedical Materials Research. Part a·Balaji V SridharKristi S Anseth
Jun 23, 2016·Advanced Healthcare Materials·Hironobu TakahashiTeruo Okano
Jun 13, 2015·Cartilage·Alan TsengCathryn A Sundback
Mar 15, 2018·Annals of Plastic Surgery·Jaime L BernsteinJason A Spector
Apr 3, 2020·Scientific Reports·Do Young ParkByung Hyune Choi
Jul 25, 2019·American Journal of Physical Medicine & Rehabilitation·Priscila Daniele de Oliveira PerruciniRodrigo Franco de Oliveira
Jan 4, 2019·Tissue Engineering and Regenerative Medicine·Long Hao JinByoung-Hyun Min

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Methods Mentioned

BETA
biopsy

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