Scaffold-free, label-free and nozzle-free biofabrication technology using magnetic levitational assembly

Biofabrication
Vladislav A ParfenovVladimir Mironov

Abstract

Tissue spheroids have been proposed as building blocks in 3D biofabrication. Conventional magnetic force-driven 2D patterning of tissue spheroids requires prior cell labeling by magnetic nanoparticles, meanwhile a label-free approach for 3D magnetic levitational assembly has been introduced. Here we present first time report on rapid assembly of 3D tissue construct using scaffold-free, nozzle-free and label-free magnetic levitation of tissue spheroids. Chondrospheres of standard size, shape and capable to fusion have been biofabricated from primary sheep chondrocytes using non-adhesive technology. Label-free magnetic levitation was performed using a prototype device equipped with permanent magnets in presence of gadolinium (Gd3+) in culture media, which enables magnetic levitation. Mathematical modeling and computer simulations were used for prediction of magnetic field and kinetics of tissue spheroids assembly into 3D tissue constructs. First, we used polystyrene beads to simulate the assembly of tissue spheroids and to determine the optimal settings for magnetic levitation in presence of Gd3+. Second, we proved the ability of chondrospheres to assemble rapidly into 3D tissue construct in the permanent magnetic field in the pr...Continue Reading

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Citations

Jul 2, 2019·Advanced Materials·Taehoon LeeBartosz A Grzybowski
Nov 28, 2019·Advanced Materials·Tanchen RenUtkan Demirci
Jun 6, 2019·Angewandte Chemie·Shencheng GeGeorge M Whitesides
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Aug 19, 2020·Advanced Healthcare Materials·Vladislav A ParfenovVladimir A Mironov
Aug 4, 2020·Science Advances·Vladislav A ParfenovVladimir A Mironov
Mar 7, 2020·Scientific Reports·Vladislav A ParfenovVladimir S Komlev
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Aug 31, 2020·Trends in Biotechnology·Ali Akbar Ashkarran, Morteza Mahmoudi
Jul 19, 2020·Applied and Environmental Microbiology·Pavel DomninSvetlana Ermolaeva
Mar 18, 2021·APL Bioengineering·Shen Ji, Murat Guvendiren
Mar 12, 2021·Cardiovascular Research·Fabian Philipp KreutzerThomas Thum
Jun 10, 2021·Communications Biology·Shreya S DeshmukhUtkan Demirci
Jul 9, 2021·Frontiers in Molecular Biosciences·Iñigo Gaitán-SalvatellaMarco Antonio Álvarez-Pérez
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Jul 15, 2020·Chemical Reviews·Mihyun LeeMarcy Zenobi-Wong
May 15, 2020·Chemical Reviews·Carlos MotaLorenzo Moroni
Oct 7, 2021·ACS Biomaterials Science & Engineering·Elizaveta V KoudanGleb B Sukhorukov

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