Scaffold-Free Tubular Tissues Created by a Bio-3D Printer Undergo Remodeling and Endothelialization when Implanted in Rat Aortae

PloS One
Manabu ItohShigeki Morita

Abstract

Small caliber vascular prostheses are not clinically available because synthetic vascular prostheses lack endothelial cells which modulate platelet activation, leukocyte adhesion, thrombosis, and the regulation of vasomotor tone by the production of vasoactive substances. We developed a novel method to create scaffold-free tubular tissue from multicellular spheroids (MCS) using a "Bio-3D printer"-based system. This system enables the creation of pre-designed three-dimensional structures using a computer controlled robotics system. With this system, we created a tubular structure and studied its biological features. Using a "Bio-3D printer," we made scaffold-free tubular tissues (inner diameter of 1.5 mm) from a total of 500 MCSs (2.5× 104 cells per one MCS) composed of human umbilical vein endothelial cells (40%), human aortic smooth muscle cells (10%), and normal human dermal fibroblasts (50%). The tubular tissues were cultured in a perfusion system and implanted into the abdominal aortas of F344 nude rats. We assessed the flow by ultrasonography and performed histological examinations on the second (n = 5) and fifth (n = 5) day after implantation. All grafts were patent and remodeling of the tubular tissues (enlargement of th...Continue Reading

References

Jan 1, 1995·Artificial Organs·Y Tomizawa
Mar 25, 1994·Science·N A Peppas, R Langer
Dec 1, 1995·Current Opinion in Cell Biology·G M Keller
Jan 10, 1998·Journal of Vascular Surgery·T C HodgesJ L Cronenwett
Jan 23, 1998·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N L'HeureuxF A Auger
Sep 14, 2000·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·P Y AoJ P Fletcher
Feb 28, 2001·The New England Journal of Medicine·T Shin'okaY Ikada
Feb 20, 2002·Trends in Neurosciences·Paul G LayerElmar Willbold
Jul 3, 2002·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·Omke E Teebken, Axel Haverich
May 21, 2003·Journal of Vascular Surgery·Sylvia E RosasHarold I Feldman
May 28, 2003·Biotechnology and Bioengineering·Jens M KelmLars K Nielsen
Mar 11, 2004·Tissue Engineering·Jens M KelmMartin Fussenegger
Jun 9, 2005·The Journal of Thoracic and Cardiovascular Surgery·Toshiharu Shin'okaHiromi Kurosawa
Jun 14, 2005·Journal of Biotechnology·Jens M KelmMartin Fussenegger
Jan 7, 2006·Circulation Research·Brett C IsenbergRobert T Tranquillo
Aug 11, 2007·Biomaterials·Peter ZillaPaul Human
Oct 5, 2007·The New England Journal of Medicine·Nicolas L'HeureuxLuis M de la Fuente
Jul 16, 2008·Journal of Cellular and Molecular Medicine·Lilian SteffensGünter Finkenzeller
Aug 5, 2008·Tissue Engineering. Part a·Carlota Diaz Sanchez-BustamanteMartin Fussenegger
Feb 14, 2009·Nature Protocols·Juergen FriedrichLeoni A Kunz-Schughart
Aug 12, 2009·Biomaterials·Cyrille NorotteGabor Forgacs
Oct 6, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Masashi YamanamiYasuhide Nakayama
May 13, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Taiji WatanabeYasuhide Nakayama
Mar 13, 2012·Biofabrication·Francoise MargaForgacs Gabor
Jun 15, 2012·Trends in Molecular Medicine·Muriel A ClearyChristopher Breuer
Nov 30, 2012·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Masashi YamanamiYasuhide Nakayama
Dec 20, 2012·Journal of Biomedicine & Biotechnology·Judee Grace Nemeno-GuanzonJeong Ik Lee
Jul 23, 2014·Biomedical Materials·L MancusoG Cao

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Citations

May 28, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Amit Panwar, Lay Poh Tan
Jan 15, 2017·Acta Biomaterialia·Pallab DattaIbrahim T Ozbolat
Dec 6, 2016·Tissue Engineering. Part B, Reviews·Nicanor I MoldovanKoichi Nakayama
Dec 8, 2016·In Vitro Cellular & Developmental Biology. Animal·Tomomi NatoriItsuko Ishii
Mar 15, 2018·Journal of Cellular and Molecular Medicine·Nicanor I Moldovan
Feb 21, 2018·Biomedical Materials·Marissa A Gionet-Gonzales, J Kent Leach
Jan 26, 2018·Advanced Healthcare Materials·Jonas C Rose, Laura De Laporte
Apr 12, 2018·Biofabrication·Xinda LiTao Xu
Jul 15, 2017·Biofabrication·Elena A BulanovaVladimir A Mironov
Sep 5, 2017·Clinical Hemorheology and Microcirculation·Anne-Helen LutterUrsula Anderer
May 26, 2018·Biofabrication·Lise De MoorHeidi Declercq
Jun 22, 2018·The Keio Journal of Medicine·Naritaka Kimura
Jan 7, 2017·Circulation Research·Anton V BorovjaginJianyi Zhang
Jan 15, 2019·Tissue Engineering. Part a·Christopher J NyczMarsha W Rolle
Sep 1, 2019·Micromachines·Wesley LaBargeJianyi Zhang
May 29, 2019·Connective Tissue Research·Shawn P GroganDarryl D D'Lima
Nov 21, 2019·The International Journal of Cardiovascular Imaging·Elodie PerdreauJean-Benoît Thambo
May 22, 2020·Biofabrication·Hongwei CaiFeng Guo
Aug 1, 2020·Advanced Healthcare Materials·Se-Jeong KimHeungsoo Shin
Aug 25, 2020·Cell Transplantation·Hirofumi YurieShuichi Matsuda
Apr 1, 2018·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Takeshi TerazawaYasuhide Nakayama
Apr 30, 2019·Small·Marcel Alexander HeinrichYu Shrike Zhang
Jul 25, 2019·Frontiers in Bioengineering and Biotechnology·Dilara Goksu TamayVasif Hasirci
Aug 17, 2019·Frontiers in Bioengineering and Biotechnology·Melanie KahlDaniel F Gilbert
Oct 1, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Saime Burce OzlerBahattin Koc
Feb 2, 2019·World Journal of Stem Cells·Shweta Anil KumarBinata Joddar
Jun 27, 2018·Innovative Surgical Sciences·Renee M MainaJohn P Geibel
Feb 23, 2020·Tissue Engineering. Part a·Yasuhiro ShudoYi-Ping Joseph Woo
Oct 22, 2016·Advanced Healthcare Materials·Jia Min Lee, Wai Yee Yeong

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Methods Mentioned

BETA
coronary artery bypass
light microscopy

Software Mentioned

Bio
Image J
- 3D
- 3D Printer
SPSS

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