Scaffold seeded with cells is essential in urothelium regeneration and tissue remodeling in vivo after bladder augmentation using in vitro engineered graft

Transplantation Proceedings
T DrewaA Wozniak

Abstract

Tissue-engineering methods using synthetic biodegradable scaffolds seeded with cells have potential to induce regeneration to a functional bladder wall. The aim of the study was to induce in vivo urothelial growth on implanted scaffolds previously seeded with stromal cells as compared with matrices implanted without cells for rat cystoplasty augmentation. 3T3 mouse fibroblasts were multiplied up to total of 10(8) cells. Cells were grown on Dulbecco's modified essential medium supplemented with 10% of fetal bovine serum and antibiotics in CO(2) chambers. Cells were seeded on biodegradable polyglycolic acid (PGA) scaffolds in eight rats: four bladders were augmented with cell-seeded grafts and the other four with acellular scaffolds. Rats were sacrificed after 4 months in preparation for hematoxylin and eosin staining. One death in the acellular cystoplasty group was observed after 3 weeks. No epithelial layer was observed in the central part of the acellular graft. The cell-seeded grafts showed good visible multilayered epithelium with at least five layers of epithelial cells in the central part. The epithelium resembled rat native urothelium. The cell-seeded grafts showed a high degree of implanted 3T3 cells infiltration with g...Continue Reading

References

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Citations

Sep 25, 2014·BioMed Research International·Silvia BaigueraCostantino Del Gaudio
Nov 27, 2008·Journal of the Royal Society, Interface·Mark R PlaczekAthanasios Mantalaris
Oct 25, 2008·Regenerative Medicine·Tomasz Drewa
Nov 9, 2011·Tissue Engineering. Part a·Abdol-Mohammad KajbafzadehMaryam Monajemzadeh
Jun 6, 2012·Transplantation Proceedings·J AdamowiczT Drewa
May 3, 2011·The Journal of Surgical Research·Lidia DapenaPilar González-Peramato
Dec 17, 2009·Transplantation Proceedings·T DrewaM Pokrywczynska
Jun 21, 2007·Transplantation Proceedings·T Drewa
Dec 6, 2014·Advanced Drug Delivery Reviews·Hsueh-Kung LinBradley P Kropp
Dec 3, 2014·Advanced Drug Delivery Reviews·Joshua R Mauney, Rosalyn M Adam
Jul 27, 2010·Surgical Innovation·Tomasz DrewaRafal Czajkowski
Mar 26, 2008·Journal of Tissue Engineering and Regenerative Medicine·Gordon R CampbellJulie H Campbell
Aug 22, 2012·Nature Reviews. Urology·Tomasz DrewaArun Sharma
Sep 19, 2015·Current Opinion in Urology·Nikolai A SopkoTrinity J Bivalacqua
Feb 6, 2017·Journal of Tissue Engineering and Regenerative Medicine·Stéphane ChabaudStéphane Bolduc

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