Scaffolding kidney organoids on silk

Journal of Tissue Engineering and Regenerative Medicine
Ashwani K GuptaLeif Oxburgh

Abstract

End stage kidney disease affects hundreds of thousands of patients in the United States. The therapy of choice is kidney replacement, but availability of organs is limited, and alternative sources of tissue are needed. Generation of new kidney tissue in the laboratory has been made possible through pluripotent cell reprogramming and directed differentiation. In current procedures, aggregates of cells known as organoids are grown either submerged or at the air-liquid interface. These studies have demonstrated that kidney tissue can be generated from pluripotent stem cells, but they also identify limitations. The first is that perfusion of cell aggregates is limited, restricting the size to which they can be grown. The second is that aggregates lack the structural integrity required for convenient engraftment and suturing or adhesion to regions of kidney injury. In this study, we evaluated the capacity of silk to serve as a support for the growth and differentiation of kidney tissue from primary cells and from human induced pluripotent stem cells. We find that cells can differentiate to epithelia characteristic of the developing kidney on this material and that these structures are maintained following engraftment under the capsu...Continue Reading

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Citations

Oct 3, 2019·Genes & Development·Melissa H Little, Alexander N Combes
May 3, 2020·Journal of the National Cancer Institute·Toni K ChoueiriHans J Hammers
Sep 20, 2020·Journal of Functional Biomaterials·Akrivi AsimakopoulouSpyros Petrakis
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Jul 10, 2019·Advanced Materials·Haitao LiuJianhua Qin
Oct 28, 2020·IET Nanobiotechnology·Anabela VeigaAna Oliveira
Feb 15, 2021·Cancer Letters·Savneet KaurAshwini Vasudevan
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Dec 9, 2019·ACS Biomaterials Science & Engineering·Kimberly J OrnellJeannine M Coburn
Nov 15, 2021·Drug Discovery Today·Chinmaya MahapatraManash K Paul

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