Apr 3, 2020

Recovery of cryo-injured rabbit urethras by biofabricated C-shaped adipose-derived mesenchymal cell structures

BioRxiv : the Preprint Server for Biology
S. Gautam SilwalOsamu Ishizuka

Abstract

Urethral tissue damage can cause stress urinary incontinence or urinary retention, and there are few long-term, effective treatments. For structural and functional recovery of urethral tissues, we designed and constructed C-shaped structures composed of adipose-derived mesenchymal cells (AMCs) by using a three-dimensional (3D)-bioprinter system, which could be transplanted in damaged urethras without obstructing the lumen. We determined if transplantation of the biofabricated structures could reconstruct the urethral tissues. AMCs were harvested from rabbits, cultured and labeled with PKH26 to form spheroids. The spheroids were assembled on a custom-designed C-shaped support by a 3D bioprinter. Urethras of rabbits were cryo-injured by spraying with liquid nitrogen for 20 seconds, incised and biofabricated structure was autologously transplanted. Control rabbits were treated similarly but without transplantation structure. Two and four weeks after surgery, the control urethras were partially constricted; however, the structure-transplanted urethras were patent. The AMCs within the structures differentiated into skeletal muscle, smooth muscle, nerve, or endothelial cells. Some cells contained growth factors and cytokines. Therefo...Continue Reading

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Mentioned in this Paper

Genome
Genome Assembly Sequence
Genomics
Sequencing
Structure
Molecular Genetic Technique
Whole Genome Sequencing
Genomic Structural Variation
Mycobacterium tuberculosis
Genome, Human

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