Scale: a chemical approach for fluorescence imaging and reconstruction of transparent mouse brain.

Nature Neuroscience
Hiroshi HamaAtsushi Miyawaki

Abstract

Optical methods for viewing neuronal populations and projections in the intact mammalian brain are needed, but light scattering prevents imaging deep into brain structures. We imaged fixed brain tissue using Scale, an aqueous reagent that renders biological samples optically transparent but completely preserves fluorescent signals in the clarified structures. In Scale-treated mouse brain, neurons labeled with genetically encoded fluorescent proteins were visualized at an unprecedented depth in millimeter-scale networks and at subcellular resolution. The improved depth and scale of imaging permitted comprehensive three-dimensional reconstructions of cortical, callosal and hippocampal projections whose extent was limited only by the working distance of the objective lenses. In the intact neurogenic niche of the dentate gyrus, Scale allowed the quantitation of distances of neural stem cells to blood vessels. Our findings suggest that the Scale method will be useful for light microscopy-based connectomics of cellular networks i...Continue Reading

References

Oct 1, 1994·Journal of Neuroscience Methods·W DenkR Yuste
Jan 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Ann-Shyn ChiangGlenn L Holbrook
Apr 16, 2003·Methods : a Companion to Methods in Enzymology·Yee-Chien Liu, Ann-Shyn Chiang
Nov 5, 2003·Nature Biotechnology·Warren R ZipfelWatt W Webb
Jul 23, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alison L BarthKathleen L Dean
Mar 23, 2005·Nature Methods·Axel NimmerjahnFritjof Helmchen
Aug 25, 2005·Applied Biochemistry and Biotechnology·Klaithem M AlkaabiS Salman Ashraf
Nov 22, 2005·Nature Methods·Fritjof Helmchen, Winfried Denk
Dec 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Giannis ZacharakisVasilis Ntziachristos
Nov 16, 2006·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Patrick Theer, Winfried Denk
Feb 6, 2007·Current Opinion in Neurobiology·Charlotta LindwallLinda J Richards
Mar 27, 2007·Cell·Hui-Hao LinAnn-Shyn Chiang
Oct 19, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Chun-Lei WangYu-Qiang Ding
Feb 13, 2008·Cell·Asako Sakaue-SawanoAtsushi Miyawaki
May 17, 2008·Development, Growth & Differentiation·Tomomi Shimogori, Masaharu Ogawa
Sep 13, 2008·Cell Stem Cell·Masoud TavazoieFiona Doetsch
Sep 20, 2008·Current Opinion in Neurobiology·Jeff W Lichtman, Joshua R Sanes
Nov 11, 2008·Neuron·Jeff W Lichtman, Stephen J Smith
Apr 14, 2009·Current Opinion in Neurobiology·Moritz HelmstaedterWinfried Denk
May 30, 2009·Nature·Erika SasakiTatsuji Nomura
Nov 20, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Philbert S TsaiDavid Kleinfeld
Jun 12, 2010·Organogenesis·Nina JährlingHans-Ulrich Dodt
Jul 10, 2010·Journal of Biomedical Optics·Sonia G ParraMichael J Levene

❮ Previous
Next ❯

Citations

Jul 19, 2013·Cellular and Molecular Life Sciences : CMLS·Chaehyun YookJinhyun Kim
Jun 27, 2013·Diabetologia·Shiue-Cheng TangYa-Yuan Fu
Mar 23, 2013·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Qian JiaoYong Liu
Sep 17, 2013·Trends in Genetics : TIG·Francesca C NorrisMark F Lythgoe
Jan 17, 2012·Nature Methods·Timothy RaganPavel Osten
Jun 19, 2012·Nature Methods·Olaf RonnebergerWolfgang Driever
Jun 1, 2013·Nature Methods·Kwanghun Chung, Karl Deisseroth
Feb 9, 2013·Microscopy·Atsushi Miyawaki
Dec 28, 2012·Journal of the Royal Society, Interface·Andreas J SchrieflGerhard A Holzapfel
Mar 29, 2013·Journal of the Royal Society, Interface·S Guenneau, T M Puvirajesinghe
May 21, 2013·Annual Review of Neuroscience·Z Josh Huang, Hongkui Zeng
Mar 17, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yuan-An LiuShiue-Cheng Tang
Nov 13, 2012·The Journal of Clinical Investigation·Christian BredeAndreas Beilhack
Aug 8, 2013·The Journal of Clinical Investigation·Christina E BarkauskasBrigid L M Hogan
Oct 5, 2013·BMC Bioinformatics·Hanchuan PengGiorgio A Ascoli
Apr 6, 2012·PloS One·Klaus BeckerHans-Ulrich Dodt
Aug 24, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Alexandra K Moore, Michael Wehr
Sep 13, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·David C RowlandClifford G Kentros
Jul 12, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gerald J SunGuo-li Ming
Feb 9, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Carlos A LafourcadeAngélique Bordey
Aug 21, 2012·Journal of the American Society of Nephrology : JASN·Robert M KaoAndrew P McMahon
Feb 22, 2013·Journal of Visualized Experiments : JoVE·Andrew J WoolleyKevin J Otto
Oct 23, 2013·Journal of Visualized Experiments : JoVE·Ludovico SilvestriFrancesco Saverio Pavone
Apr 11, 2014·Development·Alexandra D AlmeidaWilliam A Harris
Feb 27, 2014·Nature Communications·Gembu AbeKinya G Ota
Mar 19, 2014·Advanced Drug Delivery Reviews·Claudio R ThomaWilhelm Krek
Jan 1, 2013·ISRN Neuroscience·Giorgio A Ascoli
Apr 15, 2014·Frontiers in Zoology·Miguel Salinas-SaavedraAlexander O Vargas
Nov 21, 2013·Tissue Engineering. Part C, Methods·Elizabeth A CalleMichael J Levene
Jun 20, 2014·Nature Protocols·Raju TomerKarl Deisseroth
Feb 18, 2014·Advances in Colloid and Interface Science·Diogo VolpatiOsvaldo N Oliveira
Jul 9, 2014·Annual Review of Neuroscience·Scott M Sternson, Bryan L Roth
Jan 30, 2014·American Journal of Respiratory Cell and Molecular Biology·Gregory D ScottDavid B Jacoby
Jul 30, 2014·Acta Neuropathologica·Kunie AndoCharles Duyckaerts

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.