PMID: 11320509Apr 26, 2001Paper

Scale-down model to simulate spatial pH variations in large-scale bioreactors

Biotechnology and Bioengineering
A AmanullahA W Nienow

Abstract

For the first time a laboratory-scale two-compartment system was used to investigate the effects of pH fluctuations consequent to large scales of operation on microorganisms. pH fluctuations can develop in production-scale fermenters as a consequence of the combined effects of poor mixing and adding concentrated reagents at the liquid surface for control of the bulk pH. Bacillus subtilis was used as a model culture since in addition to its sensitivity to dissolved oxygen levels, the production of the metabolites, acetoin and 2,3-butanediol, is sensitive to pH values between 6.5 and 7.2. The scale-down model consisted of a stirred tank reactor (STR) and a recycle loop containing a plug flow reactor (PFR), with the pH in the stirred tank being maintained at 6.5 by addition of alkali in the loop. Different residence times in the loop simulated the exposure time of fluid elements to high values of pH in the vicinity of the addition point in large bioreactors and tracer experiments were performed to characterise the residence time distribution in it. Since the culture was sensitive to dissolved oxygen, for each experiment with pH control by adding base into the PFR, equivalent experiments were conducted with pH control by addition o...Continue Reading

References

Feb 7, 1977·Biochemical and Biophysical Research Communications·F C Stormer
Oct 1, 1973·Journal of General Microbiology·E L Speck, E Freese
May 1, 1970·European Journal of Biochemistry·D Malthe-Sorenssen, F C Stormer
May 1, 1970·European Journal of Biochemistry·J P Loken, F C Stormer
Nov 30, 1999·Biotechnology and Bioengineering·C Langheinrich, A W Nienow
Jun 22, 2000·Biotechnology and Bioengineering·F BylundG Larsson
Mar 1, 1992·Applied and Environmental Microbiology·J L TsauT J Montville
May 1, 1984·Biotechnology and Bioengineering·N M Oosterhuis, N W Kossen
May 1, 1985·Biotechnology and Bioengineering·N M OosterhuisE S Schenk
Jun 20, 1992·Biotechnology and Bioengineering·P K NamdevM R Gray

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Citations

Jul 8, 2005·Applied Microbiology and Biotechnology·F R Schmidt
Jun 20, 2007·Applied Microbiology and Biotechnology·Qiu-Li WuXue-Ming Zhao
Jul 23, 2011·Bioprocess and Biosystems Engineering·Janne WalleniusTero Eerikäinen
Sep 2, 2003·Journal of Biotechnology·Thomas Hermann
Apr 13, 2002·Current Opinion in Biotechnology·Peter HarmsGovind Rao
Jan 15, 2014·Applied Microbiology and Biotechnology·Guan WangSiliang Zhang
Oct 12, 2013·Bioprocess and Biosystems Engineering·Mohammadhadi Jazini, Christoph Herwig
Aug 25, 2015·Biotechnology and Bioengineering·José T CortésOctavio T Ramírez
Dec 31, 2011·Journal of Biotechnology·R Takors
Sep 20, 2011·Journal of Bioscience and Bioengineering·Mohammadhadi Jazini, Christoph Herwig
Feb 27, 2010·Current Opinion in Biotechnology·Peter Neubauer, Stefan Junne
Oct 23, 2010·Biotechnology and Bioengineering·Maëlle DouairePascal Loubière
Aug 18, 2009·Biotechnology and Bioengineering·Alvaro R LaraWouter A van Winden
Dec 30, 2014·Bioresource Technology·Chungman MoonMi-Sun Kim
May 5, 2011·Biotechnology Advances·R Lencastre FernandesK V Gernaey
Dec 23, 2014·Journal of Biotechnology·Benjamin NeunstoecklinMiroslav Soos
Feb 22, 2018·Biotechnology Progress·Sen XuHao Chen
Nov 20, 2018·Biotechnology and Bioengineering·Robert SpannUlrich Krühne
Mar 16, 2018·Bioprocess and Biosystems Engineering·Anna-Lena Heins, Dirk Weuster-Botz
Aug 9, 2018·Bioprocess and Biosystems Engineering·Rubin JiangSen Xu
Apr 28, 2017·Microbial Biotechnology·Joana Danica SimenUNKNOWN RecogNice-Team
Nov 10, 2020·Computational and Structural Biotechnology Journal·Jonas BisgaardKrist V Gernaey
Mar 6, 2021·Biotechnology and Bioengineering·Peng LiuHenk Noorman
Jul 8, 2017·Bioresource Technology·Yeo-Myeong YunDong-Hoon Kim

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