Abstract
Five types of dextran-based microcarriers (Dormacell(™), Pfeifer and Langen) with different concentrations of dimeric DEAE anion-exchange groups (nitrogen contents from 1.2 up to 2.9%) were tested as growth substrates for the cultivation of human umbilical vein endothelial cells (HUVECs). All microcarriers were gelatinized before use to improve cell adhesion. The one with the highest DEAE-group density was found to be most suitable for HUVEC propagation reaching final cell densities of 8×10(5) viable cells ml(-1) (95% viability) using microcarrier concentrations of 3 g l(-1). Furthermore, metabolic data of glucose/lactate and amino acid metabolism are presented in this study. The concentrations of 18 amino acids were monitored throughout cultivation. A considerable decrease of glutamine and inverse increase of glutamate was observed. Cultivation with initial glucose concentration of 16.5 mmol l(-1) resulted in high glutamine consumption rates, whereas high glucose-supplemented starting culture medium (30 mmol l(-1)) gave considerably lowered rates, indicating altered glutamine metabolism due to different glucose feeding. The glucose consumption and lactate production rates increased 2.6 fold and 3.5 fold, respectively, due to s...Continue Reading
References
Jan 1, 1991·Cytotechnology·H BüntemeyerJ Lehmann
Mar 1, 1992·Immunology Today·G A ZimmermanT M McIntyre
Nov 6, 1992·Science·L A Lasky
Feb 11, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·K WatanabeE A Jaffe
Apr 1, 1990·International Journal of Biological Macromolecules·K R JergP Friedl
May 1, 1990·Pharmaceutical Research·K L AudusR T Borchardt
Oct 1, 1990·Transplantation·J S Pober, R S Cotran
Aug 1, 1989·American Journal of Clinical Pathology·L F Fajardo
Aug 1, 1987·Archives of Biochemistry and Biophysics·B K GillardD M Marcus
Mar 1, 1987·Human Pathology·E A Jaffe
Mar 1, 1974·The Journal of Cell Biology·M A GimbroneJ Folkman
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·H Hoshi, W L McKeehan
Jan 1, 1993·Cytotechnology·G Schrimpf, P Friedl
Oct 1, 1994·Current Opinion in Cell Biology·S D Rosen, C R Bertozzi
Oct 1, 1995·Immunology Today·L D Bergelson
Jan 1, 1994·Cytotechnology·R HeidemannJ Lehmann
Aug 1, 1989·Cytotechnology·P FriedlR Czpla
Jan 1, 1995·Cytotechnology·J MüthingJ Lehmann
Citations
Mar 4, 2000·Biotechnology and Bioengineering·A WernerJ Lehmann
Nov 1, 1997·Cytotechnology·T MariqueJ Wérenne
Mar 1, 1999·Cytotechnology·E Moran
Jul 30, 2002·Glycobiology·Johannes MüthingJürgen Eck
Nov 25, 2020·International Journal of Molecular Sciences·Babak MosavatiE Du