Scale-up of hydrophobin-assisted recombinant protein production in tobacco BY-2 suspension cells

Plant Biotechnology Journal
Lauri J ReuterAnneli Ritala

Abstract

Plant suspension cell cultures are emerging as an alternative to mammalian cells for production of complex recombinant proteins. Plant cell cultures provide low production cost, intrinsic safety and adherence to current regulations, but low yields and costly purification technology hinder their commercialization. Fungal hydrophobins have been utilized as fusion tags to improve yields and facilitate efficient low-cost purification by surfactant-based aqueous two-phase separation (ATPS) in plant, fungal and insect cells. In this work, we report the utilization of hydrophobin fusion technology in tobacco bright yellow 2 (BY-2) suspension cell platform and the establishment of pilot-scale propagation and downstream processing including first-step purification by ATPS. Green fluorescent protein-hydrophobin fusion (GFP-HFBI) induced the formation of protein bodies in tobacco suspension cells, thus encapsulating the fusion protein into discrete compartments. Cultivation of the BY-2 suspension cells was scaled up in standard stirred tank bioreactors up to 600 L production volume, with no apparent change in growth kinetics. Subsequently, ATPS was applied to selectively capture the GFP-HFBI product from crude cell lysate, resulting in th...Continue Reading

References

Mar 6, 1998·Journal of Biotechnology·P M KieranD M Malone
Mar 23, 2000·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·T Nagata, F Kumagai
Nov 28, 2001·Molecular Biotechnology·R Hatti-Kaul
Nov 6, 2004·Nature Biotechnology·Stephan HellwigRainer Fischer
Feb 3, 2005·Biotechnology and Bioengineering·Andreas SchiermeyerStefan Schillberg
Dec 20, 2005·The Plant Journal : for Cell and Molecular Biology·Valerie De SutterPierre Hilson
Mar 15, 2006·Journal of Biotechnology·Kathrin SchmaleStephan Hellwig
Aug 3, 2006·Protein Science : a Publication of the Protein Society·Johanna HakanpääJuha Rouvinen
Mar 1, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Markus SackThomas Rademacher
Feb 13, 2008·Protein Expression and Purification·Tomi LahtinenChristian Oker-Blom
Jan 30, 2009·BMC Biology·Margarita TorrentM Dolors Ludevid
Sep 11, 2010·Nature Biotechnology·Gary Walsh
Jan 18, 2011·Biotechnology Advances·Jianfeng XuMaureen C Dolan
Aug 19, 2011·Biotechnology Advances·Ting-Kuo Huang, Karen A McDonald
Aug 23, 2011·Biotechnology Advances·Rainer FischerJuergen Drossard
Dec 14, 2011·Methods in Molecular Biology·Jussi J JoensuuRima Menassa
Mar 20, 2012·BMC Plant Biology·Minu JosephJean-Benoit Peltier
Dec 6, 2012·Current Medicinal Chemistry·Pauline M Doran
Jan 10, 2013·Plant Biotechnology Journal·Angelo KaldisRima Menassa
Jan 30, 2013·Molecular Biotechnology·Stefan R Schmidt

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Citations

Feb 13, 2016·Frontiers in Plant Science·Christelle M AndreGea Guerriero
Sep 4, 2015·Physiologia Plantarum·Yan-Shan DongLong-Jiang Yu
Mar 26, 2016·Frontiers in Plant Science·Rita B SantosTanja Holland
Apr 30, 2015·Biotechnology Advances·J F BuyelR Fischer
Jul 26, 2015·The Protein Journal·Mohammadreza KhalesiGuy Derdelinckx
Jan 13, 2015·Current Opinion in Biotechnology·Markus SackEva Stoger
Jan 8, 2015·Applied Microbiology and Biotechnology·Han A B Wösten, Karin Scholtmeijer
Apr 7, 2016·Annual Review of Analytical Chemistry·Marc TschofenEva Stöger
Oct 6, 2016·PloS One·Lauri ReuterJussi Joensuu
Feb 13, 2018·Frontiers in Plant Science·Suvi T HäkkinenAnneli Ritala
Jun 23, 2018·Plant Signaling & Behavior·A von SchaewenH Koiwa
Oct 18, 2019·Protein and Peptide Letters·Muhammad Sarwar KhanGhulam Mustafa
Jan 27, 2019·Journal of Biological Engineering·Bryan W Berger, Nathanael D Sallada
Jun 24, 2017·Plant Biotechnology Journal·Katri KurppaJussi J Joensuu
Jul 21, 2020·Transgenic Research·Reza Heidari-JapelaghiMokhtar Jalali-Javaran
Nov 17, 2020·Frontiers in Plant Science·Daniel TuséJohannes Felix Buyel
Apr 11, 2021·Plant Cell Reports·Uddhab KarkiJianfeng Xu
Jun 18, 2021·Frontiers in Plant Science·Bruno B SilvaMaria Izabel F Guedes
Nov 30, 2021·The Science of the Total Environment·Yumi KobayashiHanna L Tuomisto

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